| Product Number |
OAGA00887 |
| Product Page |
www.avivasysbio.com/mcm3-antibody-oaga00887.html |
| Name |
MCM3 Antibody (OAGA00887) |
| Molecular Weight |
91 kDa |
| Isotype |
IgG |
| NCBI Gene Id |
4172 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1 mg/ml |
| Gene Full Name |
DNA replication licensing factor MCM3 |
| Alias Symbols |
HCC5, P1.h, RLFB, P1-MCM3 |
| Product Format |
Liquid |
| Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-MCM3 antibody - OAGA00887 |
| Additional Information |
Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. [provided by RefSeq] |
| Additional Information |
Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji |
| Application Info |
Suggested starting dilutions are as follows: WB: 1:5000-1:20000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Immunogen |
Recombinant fragment corresponding to a region within amino acids 42 and 302 of MCM3 (Uniprot ID#P25205) |
| Additional Information |
Cellular Localization: Nucleus |
| Storage Buffer |
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Uniprot ID |
P25205 |
| Protein Name |
DNA replication licensing factor MCM3 |
| Protein Accession # |
NP_001257401.1 |
| Purification |
Purified by antigen-affinity chromatography. |
| Gene Symbol |
MCM3 |
| Predicted Species Reactivity |
Human |
| Application |
ICC, IF, IHC-FFPE, WB |
| Image 1 | HepG2
 | Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
OAGA00887 diluted at 1:10000
|
| | Image 2 | Cal27 Xenograft
 | Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using MCM3(OAGA00887) antibody at 1:500 dilution. |
| | Image 3 | HeLa
 | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCM3(OAGA00887) antibody at 1:500 dilution. |
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