| Product Number |
OAGA00757 |
| Product Page |
www.avivasysbio.com/map4k5-antibody-oaga00757.html |
| Name |
MAP4K5 Antibody (OAGA00757) |
| Molecular Weight |
95 kDa |
| Isotype |
IgG |
| NCBI Gene Id |
11183 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1 mg/ml |
| Gene Full Name |
Mitogen-activated protein kinase kinase kinase kinase 5 |
| Alias Symbols |
KHS, GCKR, KHS1, MAPKKKK5 |
| Product Format |
Liquid |
| Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-MAP4K5 antibody - OAGA00757 |
| Additional Information |
Background: This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq] |
| Additional Information |
Positive Controls: 293T, A431, H1299, HepG2, Molt-4, Raji |
| Application Info |
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Immunogen |
Synthetic peptide contain a sequence corresponding to a region within amino acids 417 and 482 of KHS |
| Additional Information |
Cellular Localization: Cytoplasm |
| Storage Buffer |
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Uniprot ID |
Q9Y4K4 |
| Protein Name |
Mitogen-activated protein kinase kinase kinase kinase 5 |
| Protein Accession # |
NP_006566.2 |
| Purification |
Purified by antigen-affinity chromatography. |
| Gene Symbol |
MAP4K5 |
| Predicted Species Reactivity |
Human |
| Application |
ICC, IF, IHC-FFPE, WB |
| Image 1 | Molt-4
 | Sample (30 ug of whole cell lysate)
A: Molt-4
12% SDS PAGE
MAP4K5 antibody
OAGA00757 diluted at 1:5000
|
| | Image 2 | SAS Xenograft
 | Immunohistochemical analysis of paraffin-embedded SAS xenograft, using KHS(OAGA00757) antibody at 1:100 dilution. |
| | Image 3 | A431
 | Immunofluorescence analysis of paraformaldehyde-fixed A431, using KHS(OAGA00757) antibody at 1:200 dilution. |
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