Product Number |
OAGA00751 |
Product Page |
www.avivasysbio.com/nhp2l1-antibody-oaga00751.html |
Name |
NHP2L1 Antibody (OAGA00751) |
Molecular Weight |
14 kDa |
Isotype |
IgG |
NCBI Gene Id |
4809 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1 mg/ml |
Gene Full Name |
NHP2-like protein 1 |
Alias Symbols |
FA1, FA-1, NHPX, 15.5K, OTK27, SSFA1, NHP2L1, SPAG12, SNRNP15-5 |
Product Format |
Liquid |
Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-NHP2L1 antibody - OAGA00751 |
Additional Information |
Background: Originally named because of its sequence similarity to the Saccharomyces cerevisiae NHP2 (non-histone protein 2), this protein appears to be a highly conserved nuclear protein that is a component of the [U4/U6.U5] tri-snRNP. It binds to the 5' stem-loop of U4 snRNA. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq] |
Additional Information |
Positive Controls: 293T, H1299, HeLa, HepG2, Molt-4, Raji |
Application Info |
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
Immunogen |
Recombinant fragment corresponding to a region within amino acids 1 and 128 of NHP2-like protein 1 (Uniprot ID#P55769) |
Additional Information |
Cellular Localization: Nucleus, nucleolus |
Storage Buffer |
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
Uniprot ID |
P55769 |
Protein Name |
NHP2-like protein 1 |
Protein Accession # |
NP_001003796.1 |
Purification |
Purified by antigen-affinity chromatography. |
Gene Symbol |
NHP2L1 |
Predicted Species Reactivity |
Human, Mouse, Rat |
Application |
ICC, IF, IHC-FFPE, WB |
Image 1 | HeLa, HepG2
| Sample (30 ug of whole cell lysate) A: HeLa B: Hep G2 12% SDS PAGE OAGA00751 diluted at 1:1000 |
| Image 2 | Neuro2A
| NHP2L1 antibody detects NHP2L1 protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug BCL-1 whole cell lysate/extract F. 30 ug Raw264.7 whole cell lysate/extract G. 30 ug C2C12 whole cell lysate/extract 15 % SDS-PAGE NHP2L1 antibody (OAGA00751) dilution: 1:1000 |
| Image 3 | PC-12
| NHP2L1 antibody detects NHP2L1 protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 15 % SDS-PAGE NHP2L1 antibody (OAGA00751) dilution: 1:1000 |
| Image 4 | Cal27 Xenograft
| Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NHP2-like protein 1(OAGA00751) antibody at 1:100 dilution. |
| Image 5 | HeLa
| Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NHP2-like protein 1(OAGA00751) antibody at 1:200 dilution. |
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