NLK Antibody (OAGA00616)

Data Sheet
Product Number OAGA00616
Product Page
Name NLK Antibody (OAGA00616)
Molecular Weight 58 kDa
Isotype IgG
NCBI Gene Id 51701
Host Rabbit
Clonality Polyclonal
Concentration 1 mg/ml
Gene Full Name Serine/threonine-protein kinase NLK
Alias Symbols DKFZp761G1211 antibody, FLJ21033 antibody, NLK antibody, serine/threonine-protein kinase NLK antibody, nemo-like kinase antibody
Product Format Liquid
Reconstitution and Storage Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-NLK antibody - OAGA00616
Additional Information Background: Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP (By similarity). Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.
Additional Information Positive Controls: A431, H1299, HeLa, mouse brain
Application Info Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunogen Recombinant fragment corresponding to a region within amino acids 234 and 527 of NLK (Uniprot ID#Q9UBE8)
Additional Information Cellular Localization: Nucleus (By similarity), Cytoplasm (By similarity)
Storage Buffer 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Uniprot ID Q9UBE8
Protein Name Serine/threonine-protein kinase NLK
Protein Accession # NP_057315.3
Purification Purified by antigen-affinity chromatography.
Gene Symbol NLK
Predicted Species Reactivity Human, Mouse
Application ICC, IF, IHC, WB
Image 1
Sample (30 ug of whole cell lysate)
A: Hela
NLK antibody
OAGA00616 diluted at 1:1000
Image 2
Mouse brain
Sample (50 ug of whole cell lysate)
A: Mouse brain
OAGA00616 diluted at 1:1000
Image 3
mouse ovary
NLK antibody [N3C3] detects NLK protein at cytosol and nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
NLK antibody [N3C3] (OAGA00616) dilution: 1:500.
Image 4
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NLK(OAGA00616) antibody at 1:100 dilution.

AVIVA SYSTEMS BIOLOGY manufactures and sells quality antibody products covering genome wide proteins.

10211 Pacific Mesa Blvd, Ste 401, San Diego, CA 92121 USA | Tel: (858)552-6979 |