| Product Number |
OAGA00616 |
| Product Page |
www.avivasysbio.com/nlk-antibody-oaga00616.html |
| Name |
NLK Antibody (OAGA00616) |
| Molecular Weight |
58 kDa |
| Isotype |
IgG |
| NCBI Gene Id |
51701 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1 mg/ml |
| Gene Full Name |
Serine/threonine-protein kinase NLK |
| Alias Symbols |
DKFZp761G1211 antibody, FLJ21033 antibody, NLK antibody, serine/threonine-protein kinase NLK antibody, nemo-like kinase antibody |
| Product Format |
Liquid |
| Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-NLK antibody - OAGA00616 |
| Additional Information |
Background: Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP (By similarity). Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3. |
| Additional Information |
Positive Controls: A431, H1299, HeLa, mouse brain |
| Application Info |
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Immunogen |
Recombinant fragment corresponding to a region within amino acids 234 and 527 of NLK (Uniprot ID#Q9UBE8) |
| Additional Information |
Cellular Localization: Nucleus (By similarity), Cytoplasm (By similarity) |
| Storage Buffer |
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Uniprot ID |
Q9UBE8 |
| Protein Name |
Serine/threonine-protein kinase NLK |
| Protein Accession # |
NP_057315.3 |
| Purification |
Purified by antigen-affinity chromatography. |
| Gene Symbol |
NLK |
| Predicted Species Reactivity |
Human, Mouse |
| Application |
ICC, IF, IHC, WB |
| Image 1 | Hela
 | Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
NLK antibody
OAGA00616 diluted at 1:1000
|
| | Image 2 | Mouse brain
 | Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
OAGA00616 diluted at 1:1000
|
| | Image 3 | mouse ovary
 | NLK antibody [N3C3] detects NLK protein at cytosol and nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
NLK antibody [N3C3] (OAGA00616) dilution: 1:500.
|
| | Image 4 | HeLa
 | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NLK(OAGA00616) antibody at 1:100 dilution. |
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