ALOXE3 Antibody (OAGA00572)

Data Sheet
 
Product Number OAGA00572
Product Page www.avivasysbio.com/aloxe3-antibody-oaga00572.html
Name ALOXE3 Antibody (OAGA00572)
Molecular Weight 81 kDa
Isotype IgG
NCBI Gene Id 59344
Host Rabbit
Clonality Polyclonal
Concentration 1 mg/ml
Gene Full Name Epidermis-type lipoxygenase 3
Alias Symbols ARCI3, E-LOX, eLOX3, eLOX-3
Product Format Liquid
Reconstitution and Storage Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-ALOXE3 antibody - OAGA00572
Additional Information Background: The leukotrienes constitute a group of arachidonic acid-derived compounds with biologic activities suggesting important roles in inflammation and immediate hypersensitivity. These compounds are catabolized, in part, by lipoxygenases. Epidermis-type lipoxygenases, a distinct subclass within the multigene family of mammalian lipoxygenases, are novel isoenzymes isolated from human and mouse skin including human ALOX15B (MIM 603697), human and mouse ALOX12B (MIM 603741), mouse 8S-LOX, and mouse e-LOX-3.[supplied by OMIM]
Additional Information Positive Controls: HepG2
Application Info Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunogen Recombinant fragment corresponding to a region within amino acids 1 and 294 of ALOXE3 (Uniprot ID#Q9BYJ1)
Storage Buffer 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Uniprot ID Q9BYJ1
Protein Name Epidermis-type lipoxygenase 3
Protein Accession # NP_001159432.1
Purification Purified by antigen-affinity chromatography.
Gene Symbol ALOXE3
Predicted Species Reactivity Human
Application IHC-FFPE, WB
Image 1
HepG2
Sample(30 ug whole cell lysate)
A:Hep G2
7.5% SDS PAGE
OAGA00572 diluted at 1:1000
Image 2
NCI-N87 Xenograft
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using ALOXE3(OAGA00572) antibody at 1:500 dilution.
 

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