VTA1 Antibody (OAGA00570)

Data Sheet
 
Product Number OAGA00570
Product Page www.avivasysbio.com/vta1-antibody-oaga00570.html
Name VTA1 Antibody (OAGA00570)
Molecular Weight 34 kDa
Isotype IgG
NCBI Gene Id 51534
Host Rabbit
Clonality Polyclonal
Concentration 0.74 mg/ml
Gene Full Name Vacuolar protein sorting-associated protein VTA1 homolog
Alias Symbols DRG1, LIP5, SBP1, DRG-1, My012, 6orf55, C6orf55, HSPC228
Product Format Liquid
Reconstitution and Storage Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-VTA1 antibody - OAGA00570
Additional Information Background: C6ORF55 encodes a protein involved in trafficking of the multivesicular body, an endosomal compartment involved in sorting membrane proteins for degradation in lysosomes (Ward et al., 2005 [PubMed 15644320]).[supplied by OMIM]
Additional Information Positive Controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji
Application Info Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunogen Recombinant fragment corresponding to a region within amino acids 1 and 276 of VTA1 (Uniprot ID#Q9NP79)
Additional Information Cellular Localization: Cytoplasm, Endosome membrane; Peripheral membrane protein
Storage Buffer 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Uniprot ID Q9NP79
Protein Name Vacuolar protein sorting-associated protein VTA1 homolog
Protein Accession # NP_057569.2
Purification Purified by antigen-affinity chromatography.
Gene Symbol VTA1
Predicted Species Reactivity Human
Application ICC, IF, IHC-FFPE, WB
Image 1
H1299, HeLa S3, MOLT4
Sample(30 ug whole cell lysate)
A:H1299
B:HeLa S3
C:MOLT4
12% SDS PAGE
OAGA00570 diluted at 1:1000
Image 2
SW480 Xenograft
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using VTA1(OAGA00570) antibody at 1:500 dilution.
Image 3
A431
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using VTA1(OAGA00570) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with(Red) at 1:500.
 

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