| Product Number |
OAGA00556 |
| Product Page |
www.avivasysbio.com/gle1-antibody-oaga00556.html |
| Name |
GLE1 Antibody (OAGA00556) |
| Molecular Weight |
75 kDa |
| Isotype |
IgG |
| NCBI Gene Id |
2733 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1 mg/ml |
| Gene Full Name |
Nucleoporin GLE1 |
| Alias Symbols |
LCCS, CAAHC, CAAHD, GLE1L, LCCS1, hGLE1 |
| Product Format |
Liquid |
| Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-GLE1 antibody - OAGA00556 |
| Additional Information |
Background: This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Additional Information |
Positive Controls: 293T, A431, HeLa, Molt-4, Raji |
| Application Info |
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Immunogen |
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 414 and 646 of GLE1 |
| Additional Information |
Cellular Localization: Nucleus, Cytoplasm, Isoform 1: Cytoplasm, nuclear pore complex |
| Storage Buffer |
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Uniprot ID |
Q53GS7 |
| Protein Name |
Nucleoporin GLE1 |
| Protein Accession # |
NP_001003722.1 |
| Purification |
Purified by antigen-affinity chromatography. |
| Gene Symbol |
GLE1 |
| Predicted Species Reactivity |
Human |
| Application |
ICC, IF, IHC-FFPE, WB |
| Image 1 | HeLa
 | Sample (30 ug of whole cell lysate)
A: HeLa
7.5% SDS PAGE
OAGA00556 diluted at 1:1000 |
| | Image 2 | Cal27 Xenograft
 | Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using GLE1(OAGA00556) antibody at 1:500 dilution. |
| | Image 3 | HeLa
 | Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GLE1(OAGA00556) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with(Red) at 1:2500. |
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