ZKSCAN3 Antibody (OAGA00456)

Data Sheet
 
Product Number OAGA00456
Product Page www.avivasysbio.com/zkscan3-antibody-oaga00456.html
Name ZKSCAN3 Antibody (OAGA00456)
Molecular Weight 61 kDa
Isotype IgG
NCBI Gene Id 80317
Host Rabbit
Clonality Polyclonal
Concentration 1 mg/ml
Gene Full Name Zinc finger protein with KRAB and SCAN domains 3
Alias Symbols ZF47, Zfp47, ZFP306, ZNF306, ZNF309, zfp-47, ZSCAN13, ZSCAN35, dJ874C20.1, dJ874C20.1.
Product Format Liquid
Reconstitution and Storage Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-ZKSCAN3 antibody - OAGA00456
Additional Information Background: Acts as a transcriptional regulator. Binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3'. Associates with chromatin at the ITGB4 and VEGF promoters. Activates the transcription of genes associated with colon cancer progression.
Additional Information Positive Controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji
Application Info Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunogen Recombinant fragment corresponding to a region within amino acids 1 and 394 of ZKSCAN3 (Uniprot ID#Q9BRR0)
Storage Buffer 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Uniprot ID Q9BRR0
Protein Name Zinc finger protein with KRAB and SCAN domains 3
Protein Accession # NP_001229823.1
Purification Purified by antigen-affinity chromatography.
Gene Symbol ZKSCAN3
Predicted Species Reactivity Human
Application ICC, IF, IHC-FFPE, WB
Image 1
HeLa S3, HepG2
Sample(30 ug whole cell lysate)
A:HeLa S3
B:Hep G2
7.5% SDS PAGE
OAGA00456 diluted at 1:1000
Image 2
normal lung
Immunohistochemical analysis of paraffin-embedded normal lung, using ZKSCAN3(OAGA00456) antibody at 1:100 dilution.
Image 3
HeLa
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZNF306 (OAGA00456) antibody at 1:200 dilution.
 

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