| Product Number |
OAGA00423 |
| Product Page |
www.avivasysbio.com/nkx2-5-antibody-oaga00423.html |
| Name |
NKX2-5 Antibody (OAGA00423) |
| Molecular Weight |
35 kDa |
| Isotype |
IgG |
| NCBI Gene Id |
1482 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1 mg/ml |
| Gene Full Name |
Homeobox protein Nkx-2.5 |
| Alias Symbols |
CSX, CSX1, VSD3, CHNG5, HLHS2, NKX2E, NKX2.5, NKX4-1 |
| Product Format |
Liquid. PBS containing 20% glycerol and 0.025% Proclin300. |
| Description of Target |
Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.[supplied by OMIM] |
| Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-NKX2-5 antibody - OAGA00423 |
| Application Info |
WB 1:500~3000
ICC/IF 1:100~1000.
Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Immunogen |
Recombinant fragment corresponding to a region within amino acids 1 and 218 of Nkx2.5 (Uniprot ID#P52952) |
| Additional Information |
Cellular Localization: Nucleus |
| Uniprot ID |
P52952 |
| Protein Name |
Homeobox protein Nkx-2.5 |
| Protein Accession # |
NP_001159647.1 |
| Purification |
Purified by antigen-affinity chromatography. |
| Gene Symbol |
NKX2-5 |
| Predicted Species Reactivity |
Human, Mouse |
| Application |
ICC, IF, WB |
| Predicted Homology Based on Immunogen Sequence |
Rat, Dog, Bovine |
| Image 1 | H1299, Raji
 | Sample(30 ug whole cell lysate)
A:H1299
B:Raji
10% SDS PAGE
OAGA00423 diluted at 1:1000 |
| | Image 2 | mouse heart
 | Sample (50 ug of whole cell lysate)
A: mouse heart
10% SDS PAGE
OAGA00423 diluted at 1:1000
|
| | Image 3 | rat heart
 | Nkx2.5 antibody detects NKX2-5 protein by Western blot analysis.
A. 50 ug rat heart lysate/extract
10 % SDS-PAGE
Nkx2.5 antibody (OAGA00423) dilution: 1:500 |
| | Image 4 | HeLa
 | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NKX2-5(OAGA00423) antibody at 1:200 dilution. |
| | Image 5 | mouse ESC D3
 | Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Nkx2.5(OAGA00423) antibody at 1:200 dilution. |
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