Product Number |
OAGA00185 |
Product Page |
www.avivasysbio.com/dsg2-antibody-oaga00185.html |
Name |
DSG2 Antibody (OAGA00185) |
Molecular Weight |
122 kDa |
Isotype |
IgG |
NCBI Gene Id |
1829 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1 mg/ml |
Gene Full Name |
Desmoglein-2 |
Alias Symbols |
HDGC, CDHF5 |
Product Format |
Liquid |
Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-DSG2 antibody - OAGA00185 |
Additional Information |
Background: Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. This gene product is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines. Mutations in this gene have been associated with arrhythmogenic right ventricular dysplasia, familial, 10. [provided by RefSeq] |
Additional Information |
Positive Controls: 293T, H1299, Molt-4 |
Application Info |
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
Immunogen |
Recombinant fragment corresponding to a region within amino acids 1 and 206 of Desmoglein 2 (Uniprot ID#Q14126) |
Additional Information |
Cellular Localization: Cell membrane, Cell junction, desmosome |
Storage Buffer |
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
Uniprot ID |
Q14126 |
Protein Name |
Desmoglein-2 |
Protein Accession # |
NP_001934.2 |
Purification |
Purified by antigen-affinity chromatography. |
Gene Symbol |
DSG2 |
Predicted Species Reactivity |
Human |
Application |
ICC, IF, IHC-FFPE, WB |
Image 1 | 293T , H1299 , Molt-4
| Sample (30 ug of whole cell lysate) A: 293T B: H1299 C: Molt-4 7.5% SDS PAGE OAGA00185 diluted at 1:1000
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| Image 2 | DU145 Xenograft
| Immunohistochemical analysis of paraffin-embedded DU145 xenograft, using Desmoglein-2(OAGA00185) antibody at 1:100 dilution. |
| Image 3 | A431
| Immunofluorescence analysis of paraformaldehyde-fixed A431, using Desmoglein 2(OAGA00185) antibody at 1:500 dilution. |
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