RAD9A Antibody (OAGA00007)

Data Sheet
 
Product Number OAGA00007
Product Page www.avivasysbio.com/rad9a-antibody-oaga00007.html
Name RAD9A Antibody (OAGA00007)
Molecular Weight 43 kDa
Isotype IgG
NCBI Gene Id 5883
Host Rabbit
Clonality Polyclonal
Concentration 1 mg/ml
Gene Full Name Cell cycle checkpoint control protein RAD9A
Alias Symbols RAD9
Product Format Liquid
Reconstitution and Storage Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-RAD9A antibody - OAGA00007
Additional Information Background: This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene. [provided by RefSeq]
Additional Information Positive Controls: A431, HeLa, C2C12, Rat2
Application Info Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunogen Recombinant fragment contain a sequence corresponding to a region within amino acids 197 and 391 of Rad9
Additional Information Cellular Localization: Nucleus
Storage Buffer 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Uniprot ID Q99638
Protein Name Cell cycle checkpoint control protein RAD9A
Protein Accession # NP_001230153.1
Purification Purified by antigen-affinity chromatography.
Gene Symbol RAD9A
Predicted Species Reactivity Human, Mouse, Rat
Application ICC, IF, IHC-FFPE, WB
Image 1
A431, Hela
Sample (30 ug of whole cell lysate)
A: A431
B: Hela
10% SDS PAGE
OAGA00007 diluted at 1:1000
Image 2
C2C12
Rad9 antibody detects RAD9A protein by Western blot analysis.
A. 30 ug C2C12 whole cell lysate/extract
10 % SDS-PAGE
Rad9 antibody (OAGA00007) dilution: 1:500
Image 3
Rat2
Rad9 antibody detects RAD9A protein by Western blot analysis.
A. 30 ug Rat2 whole cell lysate/extract
10 % SDS-PAGE
Rad9 antibody (OAGA00007) dilution: 1:1000
Image 4
HBL435 Xenograft
Rad9 antibody detects RAD9A protein at nucleus on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
Rad9 antibody (OAGA00007) dilution: 1:500.
Image 5
HeLa
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Rad9 (OAGA00007) antibody at 1:100 dilution.
 

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