Product Number |
OAGA00007 |
Product Page |
www.avivasysbio.com/rad9a-antibody-oaga00007.html |
Name |
RAD9A Antibody (OAGA00007) |
Molecular Weight |
43 kDa |
Isotype |
IgG |
NCBI Gene Id |
5883 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1 mg/ml |
Gene Full Name |
Cell cycle checkpoint control protein RAD9A |
Alias Symbols |
RAD9 |
Product Format |
Liquid |
Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-RAD9A antibody - OAGA00007 |
Additional Information |
Background: This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene. [provided by RefSeq] |
Additional Information |
Positive Controls: A431, HeLa, C2C12, Rat2 |
Application Info |
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
Immunogen |
Recombinant fragment contain a sequence corresponding to a region within amino acids 197 and 391 of Rad9 |
Additional Information |
Cellular Localization: Nucleus |
Storage Buffer |
1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
Uniprot ID |
Q99638 |
Protein Name |
Cell cycle checkpoint control protein RAD9A |
Protein Accession # |
NP_001230153.1 |
Purification |
Purified by antigen-affinity chromatography. |
Gene Symbol |
RAD9A |
Predicted Species Reactivity |
Human, Mouse, Rat |
Application |
ICC, IF, IHC-FFPE, WB |
Image 1 | A431, Hela
| Sample (30 ug of whole cell lysate) A: A431 B: Hela 10% SDS PAGE OAGA00007 diluted at 1:1000
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| Image 2 | C2C12
| Rad9 antibody detects RAD9A protein by Western blot analysis. A. 30 ug C2C12 whole cell lysate/extract 10 % SDS-PAGE Rad9 antibody (OAGA00007) dilution: 1:500 |
| Image 3 | Rat2
| Rad9 antibody detects RAD9A protein by Western blot analysis. A. 30 ug Rat2 whole cell lysate/extract 10 % SDS-PAGE Rad9 antibody (OAGA00007) dilution: 1:1000 |
| Image 4 | HBL435 Xenograft
| Rad9 antibody detects RAD9A protein at nucleus on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. Rad9 antibody (OAGA00007) dilution: 1:500.
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| Image 5 | HeLa
| Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Rad9 (OAGA00007) antibody at 1:100 dilution. |
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