PRKACB Antibody (OAEE00519)

• Product Number: OAEE00519
• Name: PRKACB Antibody (OAEE00519)
• Clone: 6D2.1
• Isotype: IgG1
• NCBI Gene Id: 5566
• Host: Mouse
• Clonality: Monoclonal
• Purity: > 95% (by SDS-PAGE)
• Concentration: 1 mg/ml
• Gene Full Name: protein kinase cAMP-activated catalytic subunit alpha
• Alias Symbols: CAFD1;cAMP-dependent protein kinase catalytic subunit alpha;PKA C-alpha;PKACA;PPNAD4;protein kinase A catalytic subunit;protein kinase, cAMP-dependent, alpha catalytic subunit;protein kinase, cAMP-dependent, catalytic, alpha.
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Reference: Komagiri Y, Kitamura N.: Comparison of effects of PKA catalytic subunit on I(h) and calcium channel currents in rat dorsal root ganglion cells. Biomed Res. 2007 Aug;28(4):177-89.
  Ferraris JD, Persaud P, Williams CK, Chen Y, Burg M.B.: cAMP-independent role of PKA in tonicity-induced transactivation of tonicity-responsive enhancer/ osmotic response element-binding protein. Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16800-5.
  Doucas V, Shi Y, Miyamoto S, West A, Verma I, Evans R.M.: Cytoplasmic catalytic subunit of protein kinase A mediates cross-repression by NF-kappa B and the glucocorticoid receptor. Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):11893-8.
  Beebe SJ, Oyen O, Sandberg M, Frøysa A, Hansson V, Jahnsen T.: Molecular cloning of a tissue-specific protein kinase (C gamma) from human testis--representing a third isoform for the catalytic subunit of cAMP-dependentprotein kinase. Mol Endocrinol. 1990 Mar;4(3):465-75.
  Kiger JA Jr, Eklund JL, Younger SH, O'Kane C.J.: Transgenic inhibitors identify two roles for protein kinase A in Drosophiladevelopment. Genetics. 1999 May;152(1):281-90.
• Description of Target: Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca(2+), leading to reduced amplitude and increased frequency of store overload-induced Ca(2+) release (SOICR) characterized by an increased rate of Ca(2+) release and propagation velocity of spontaneous Ca(2+) waves, despite reduced wave amplitude and resting cytosolic Ca(2+). PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT). Phosphorylates APOBEC3G and AICDA. Isoform 2 phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation. Phosphorylates HSF1; this phosphorylation promotes HSF1 nuclear localization and transcriptional activity upon heat shock (PubMed:21085490).
• Reconstitution and Storage: 2°C to 8°C
• Specificity: The antibody 6D2.1 strongly reacts with human proteinkinase A catalytic (PKAc) alpha subunit, and weakly with PKAc gamma subunit (both around 40 kDa). The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog.
• Immunogen: Peptide corresponding to amino acids ESPAQNTAHLDQFERIK of human proteinkinase A c alpha (PKAc alpha).
• Formulation: Purified
• Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Uniprot ID: P17612
• Protein Name: cAMP-dependent protein kinase catalytic subunit alpha
• Protein Accession #: NP_001229786.1; NP_001229787.1; NP_001229788.1; NP_001229789.1; NP_001229790.1; NP_001229791.1
• Purification: Purified
• Nucleotide Accession #: NM_00124; NM_001242857.2; NM_001242858.2; NM_001242859.2; NM_001242860.2; NM_001242861.2
• Gene Symbol: PRKACA
• Predicted Species Reactivity: Human
• Application: Western blot
• Image 1: GST fusion proteins
  
  Dot Blot analysis of GST and GST-fusion proteins using anti-PKAc (6D2.1) and anti-GST (S-tag-05).
  The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot.
  Lane 1: GST-Akt1
  Lane 2: GST-Akt2
  Lane 3: GST-Akt3
  Lane 4: GST-PKAc alpha
  Lane 5: GST-PKAc beta
  Lane 6: GST-PKAc gamma
  Lane 7: GST-MEK 1
  Lane 8: GST