| Product Number |
OAEE00057 |
| Product Page |
www.avivasysbio.com/cd117-antibody-oaee00057.html |
| Name |
CD117 Antibody (OAEE00057) |
| Clone |
104D2 |
| Isotype |
IgG1 |
| Conjugation |
Biotin |
| NCBI Gene Id |
3815 |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Concentration |
1 mg/ml |
| Gene Full Name |
KIT proto-oncogene, receptor tyrosine kinase |
| Description |
|
| Alias Symbols |
CD117;C-Kit;c-Kit protooncogene;mast/stem cell growth factor receptor Kit;MASTC;p145 c-kit;PBT;piebald trait protein;proto-oncogene c-Kit;proto-oncogene tyrosine-protein kinase Kit;SCFR;soluble KIT variant 1;tyrosine-protein kinase Kit;v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog;v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein. |
| Product Format |
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Reference |
Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
Stevenson KS, Mc Glynn L, Hodge M, Mc Linden H, George WD, Davies RW, Shiels PG: Isolation, Characterisation and Differentiation of Thy1.1 sorted Pancreatic Adult Progenitor Cell Populations. Stem Cells Dev. 2009 Mar 27.
Nagano M, Yamashita T, Hamada H, Ohneda K, Kimura K, Nakagawa T, Shibuya M, Yoshikawa H, Ohneda O: Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood. Blood. 2007 Jul 1;110(1):151-60.
Wihlidal P, Varga F, Pfeilstöcker M, Karlic H: Expression and functional significance of osteocalcin splicing in disease progression of hematological malignancies. Leuk Res. 2006 Oct;30(10):1241-8.
Blair A, Sutherland HJ: Primitive acute myeloid leukemia cells with long-term proliferative ability in vitro and in vivo lack surface expression of c-kit (CD117). Exp Hematol. 2000 Jun;28(6):660-71.
Yoshino N, Ami Y, Terao K, Tashiro F, Honda M: Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp Anim. 2000 Apr;49(2):97-110.
Broudy VC, Lin NL, Liles WC, Corey SJ, O'Laughlin B, Mou S, Linnekin D: Signaling via Src family kinases is required for normal internalization of the receptor c-Kit. Blood. 1999 Sep 15;94(6):1979-86.
Broudy VC, Lin NL, Bühring HJ, Komatsu N, Kavanagh TJ: Analysis of c-kit receptor dimerization by fluorescence resonance energytransfer. Blood. 1998 Feb 1;91(3):898-906.
Rappold I, Ziegler BL, Köhler I, Marchetto S, Rosnet O, Birnbaum D, Simmons PJ, Zannettino AC, Hill B, Neu S, Knapp W, Alitalo R, Alitalo K, Ullrich A, Kanz L, Bühring HJ: Functional and phenotypic characterization of cord blood and bone marrow subsets expressing FLT3 (CD135) receptor tyrosine kinase. Blood. 1997 Jul 1;90(1):111-25.
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| Description of Target |
Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. |
| Reconstitution and Storage |
2°C to 8°C |
| Datasheets/Manuals |
Printable datasheet for CD117 Antibody (OAEE00057) (OAEE00057) |
| Specificity |
The mouse monoclonal antibody 104D2 detects extracellular part of CD117 / c-Kit protooncogen.
HLDA VI; WS Code C-30 |
| Immunogen |
MOLM-1 megakaryocytic cells |
| Formulation |
Biotin |
| Storage Buffer |
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Uniprot ID |
P10721 |
| Protein Name |
Mast/stem cell growth factor receptor Kit |
| Publications |
Expression of Luminal Progenitor Marker CD117 in the Human Breast Gland. J Histochem Cytochem. 66, 879-888 (2018). 30004288 |
| Protein Accession # |
NP_000213.1; NP_001087241.1 |
| Purification |
Purified by size-exclusion chromatography |
| Nucleotide Accession # |
NM_000222.2; NM_001093772.1 |
| Gene Symbol |
KIT |
| Predicted Species Reactivity |
Bovine|Human|Non-human Primate |
| Application |
Flow cytometry|Immunocytochemistry|Immunohistochemistry-Frozen|Immunoprecipitation |
| Image 1 | Human PBL
 | Surface staining of human peripheral blood cells with anti-CD117 (104D2) purified. |
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