IRS-1 Antibody (Phospho-Ser1101) (OAEC01912)

• Product Number: OAEC01912
• Name: IRS-1 Antibody (Phospho-Ser1101) (OAEC01912)
• Molecular Weight: 132 kDa
• NCBI Gene Id: 3667
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Gene Full Name: Insulin receptor substrate 1
• Alias Symbols: HIRS-1
• Product Format: Liquid
• Reference: XJ Sun, J. Biol. Chem., Nov 1992; 267: 22662 - 22672.Laurent Delahaye, Endocrinology, Dec 1998; 139: 4911.Angela M. Valverde, Mol. Cell. Biol., Apr 2001; 21: 2269 - 2280. Xiao Jian Sun, J. Biol. Chem., May 1996; 271: 10583.
• Reconstitution and Storage: Store at -20C/1 year
• Specificity: IRS-1 (Phospho-Ser1101) antibody detects endogenous levels of IRS-1 only whenphosphorylated at serine 1101.
• Additional Information: All site: Human: Ser1101; Mouse: Ser1097; Rat: Ser1100
• Application Info: WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:5000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from humanIRS-1 around the phosphorylation site of serine 1101 (H-S-S^P -E-T).
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Uniprot ID: P35568
• Protein Name: Insulin receptor substrate 1
• Protein Accession #: NP_005535.1
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation
• Gene Symbol: IRS1
• Predicted Species Reactivity: Human, Mouse, Rat
• Application: WB, IHC
• Image 1: COS-7
  
  Western blot analysis of extracts from COS7 cells, treatedwith Calyculin A (50ng/m l, 30mins), using IRS-1 (Phospho-Ser1101) antibody.
• Image 2: Human colon carcinoma
  
  Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using IRS-1(Phospho-Ser1101) antibody.