| Product Number |
OAEC00165 |
| Product Page |
www.avivasysbio.com/pkc-antibody-phospho-thr641-oaec00165.html |
| Name |
PKCb Antibody (Phospho-Thr641) (OAEC00165) |
| Molecular Weight |
77 kDa |
| NCBI Gene Id |
5579 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1 mg/ml |
| Gene Full Name |
Protein kinase C beta type |
| Alias Symbols |
PKCB, PRKCB1, PRKCB2, PKCI(2), PKCbeta, PKC-beta |
| Product Format |
Liquid |
| Reference |
Zhang Y, et al. (2006) Mol Cell Biol ; 26: 6748-6761 Castoria G, et al. (2004) Mol Cell Biol ; 24: 7643-7653 Marcil J, et al. (1999) Biochem J ; 337:185-192 Bornancin F, et al. (1996) Curr Biol ; 6:1114-1123. |
| Reconstitution and Storage |
Store at -20C/1 year |
| Datasheets/Manuals |
Printable datasheet for anti-PRKCB antibody OAEC00165 |
| Specificity |
PKC? (phospho-Thr641) antibody detects endogenous levels of PKC? only when phosphorylated at threonine 641. |
| Application Info |
WB: 1:500-1:1000, IHC: 1:50-1:100 |
| Immunogen |
The antiserum was produced against synthesized phosphopeptide derived from human PKC? around the phosphorylation site of threonine 641 (E-L-TP-P-T). |
| Formulation |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Uniprot ID |
P05771 |
| Protein Name |
Protein kinase C beta type |
| Protein Accession # |
NP_002729.2 |
| Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation |
| Gene Symbol |
PRKCB |
| Predicted Species Reactivity |
Human, Mouse, Rat |
| Application |
IF, IHC, WB |
| Image 1 | Human lung carcinoma
 | Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using PKCβ (phospho-Thr641) antibody (OAEC00165). |
| | Image 2 | K562
 | Western blot analysis of extracts from K562 cells, untreated or treated with PMA (1ng/ml, 10min), using PKCβ (Ab-641) antibody (OAEC00526, Lane 1 and 2) and PKCβ (phospho-Thr641) antibody (OAEC00165, Lane 3 and 4). |
| | Image 3 | MCF-7
 | Immunofluroescence staining of methanol-fixed MCF7 cells usingPRKCB Antibody (OAEC00165, Red) |
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