PLC?1 Antibody (Phospho-Tyr783) (OAEC00101)

Data Sheet
 
Product Number OAEC00101
Product Page www.avivasysbio.com/plc-1-antibody-phospho-tyr783-oaec00101.html
Name PLC?1 Antibody (Phospho-Tyr783) (OAEC00101)
Molecular Weight 149 kDa
NCBI Gene Id 5335
Host Rabbit
Clonality Polyclonal
Concentration 1 mg/ml
Gene Full Name 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
Alias Symbols PLC1, NCKAP3, PLC-II, PLC148, PLCgamma1
Product Format Liquid
Reference DeBell KE, et al. Mol Cell Biol. 1999 Nov; 19(11): 7388-7398. VerĂ­ MC, et al. Mol Cell Biol. 2001 Oct; 21(20): 6939-6950. Sette C, et al. EMBO J. 2002 Oct 15; 21(20): 5386-5395.
Reconstitution and Storage Store at -20C/1 year
Datasheets/Manuals Printable datasheet for anti-PLCG1 antibody OAEC00101
Specificity PLC?1 (phospho-Tyr783) antibody detects endogenous levels of PLC?1 only when phosphorylated at tyrosine 783.
Application Info WB: 1:500-1:1000
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PLC?1 around the phosphorylation site of tyrosine 783 (G-F-YP-V-E).
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Uniprot ID P19174
Protein Name 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
Protein Accession # NP_002651.2
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation
Gene Symbol PLCG1
Predicted Species Reactivity Human, Mouse, Rat
Application WB
Image 1
A431
Western blot analysis of extract from A431 cells, untreated or treated with EGF (200ng/ml, 10min), using PLCγ1 (Ab-783) antibody (OAEC00477, Lane 1 and 2) and PLCγ1 (phospho-Tyr783) antibody (OAEC00101, Lane 3 and 4).
 

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