PKD/PKCu Antibody (Phospho-Ser910) (OAEC00094)

• Product Number: OAEC00094
• Name: PKD/PKCu Antibody (Phospho-Ser910) (OAEC00094)
• Molecular Weight: 102 kDa
• NCBI Gene Id: 5587
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Gene Full Name: Serine/threonine-protein kinase D1
• Alias Symbols: PKD, PKCM, CHDED, PRKCM, PKC-MU
• Product Format: Liquid
• Reference: Matthews SA, et al. J Biol Chem 1999 Sep; 274(37): 26543-26549 Brandlin I, et al. J Biol Chem 2002 Feb; 277(8): 6490-6496 Storz P, et al. Mol Pharmacol 2004 Oct; 66(4): 870-879
• Reconstitution and Storage: Store at -20C/1 year
• Specificity: PKD/PKCu (phospho-Ser910) antibody detects endogenous levels of PKD/PKCu only when phosphorylated at serine 910.
• Application Info: WB: 1:500-1:1000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human PKD/PKCu around the phosphorylation site of serine 910 (R-V-S^P-I-L).
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Uniprot ID: Q15139
• Protein Name: Serine/threonine-protein kinase D1
• Protein Accession #: NP_002733.2
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation
• Gene Symbol: PRKD1
• Predicted Species Reactivity: Human, Mouse, Rat
• Application: IF, WB
• Image 1: A431
  
  Western blot analysis of extract from A431 cells, untreated or treated with EGF (200ng/ml, 10min), using PKD/PKCu (Ab-910) antibody (OAEC00476, Lane 1 and 2) and PKD/PKCu (phospho-Ser910) antibody (OAEC00094, Lane 3 and 4).
• Image 2: HeLa
  
  Immunofluroescence staining of methanol-fixed HeLa cells using PRKD1 Antibody (OAEC00094, Red)