PKD/PKCu Antibody (Phospho-Ser738) (OAEC00077)

• Product Number: OAEC00077
• Name: PKD/PKCu Antibody (Phospho-Ser738) (OAEC00077)
• Molecular Weight: 102 kDa
• NCBI Gene Id: 5587
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Gene Full Name: Serine/threonine-protein kinase D1
• Alias Symbols: PKD, PKCM, CHDED, PRKCM, PKC-MU
• Product Format: Liquid
• Reference: Storz P, et al. Mol Cell Biol. 2004 Apr; 24(7): 2614-2626. Storz P, et al. Mol Cell Biol. 2005 Oct; 25(19): 8520-8530. Zhang W, et al. J Biol Chem 2005 May 13; 280(19): 19036-19044
• Reconstitution and Storage: Store at -20C/1 year
• Specificity: PKD/PKCu (phospho-Ser738) antibody detects endogenous levels of PKD/PKCu only when phosphorylated at serine 738.
• Application Info: WB: 1:500-1:1000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human PKD/PKCu around the phosphorylation site of serine 738 (E-K-S^P-F-R).
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Uniprot ID: Q15139
• Protein Name: Serine/threonine-protein kinase D1
• Protein Accession #: NP_002733.2
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation
• Gene Symbol: PRKD1
• Predicted Species Reactivity: Human, Mouse, Rat
• Application: IF, WB
• Image 1: A431
  
  Western blot analysis of extract from A431 cells, untreated or treated with EGF (200ng/ml, 10min), using PKD/PKCu (Ab-738) antibody (OAEC00474, Lane 1 and 2) and PKD/PKCu (phospho-Ser738) antibody (OAEC00077, Lane 3 and 4).
• Image 2: HeLa
  
  Immunofluroescence staining of methanol-fixed HeLa cells using PRKD1 Antibody (OAEC00077, Red)