HER2 Antibody (Phospho-Tyr877) (OAEC00074)

• Product Number: OAEC00074
• Name: HER2 Antibody (Phospho-Tyr877) (OAEC00074)
• Molecular Weight: 138 kDa
• NCBI Gene Id: 2064
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Gene Full Name: Receptor tyrosine-protein kinase erbB-2
• Alias Symbols: NEU, NGL, HER2, TKR1, CD340, HER-2, MLN 19, HER-2/neu
• Product Format: Liquid
• Reference: Dittadi, R. et al. (2000) J. Natl. Cancer Inst. 92, 1443-1444.
  Muthuswamy, S. K. et al. (1999) Mol. Cell. Biol. 19, 6845-6857.
  Qian, X. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 1500-1504.
• Reconstitution and Storage: Store at -20C/1 year
• Specificity: HER2 (phospho-Tyr877) antibody detects endogenous levels of HER2 only when phosphorylated at tyrosine 877.
• Application Info: WB: 1:500-1:1000, IHC: 1:50-1:100, IF:1:100-1:200
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human HER2 around the phosphorylation site of tyrosine 877 (T-E-Y^P-H-A).
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Uniprot ID: P04626
• Protein Name: Receptor tyrosine-protein kinase erbB-2
• Protein Accession #: NP_001005862.1
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Gene Symbol: ERBB2
• Predicted Species Reactivity: Human, Mouse, Rat
• Application: WB, IHC, IF
• Image 1: Human breast carcinoma
  
  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HER2 (phospho- Tyr877) antibody (OAEC00074).
• Image 2: MDA-MB-231
  
  Western blot analysis of extract from MDA-MB-231 cells treated or untreated with EGF using HER2 (Ab-877) Antibody (OAEC00420, Line 1 and 2) and HER2 (phospho-Tyr877) antibody (OAEC00074, Line 3 and 4).