Estrogen Receptor-a Antibody (Phospho-Ser106) (OAEC00070)

Data Sheet
 
Product Number OAEC00070
Product Page www.avivasysbio.com/estrogen-receptor-antibody-phospho-ser106-oaec00070.html
Name Estrogen Receptor-a Antibody (Phospho-Ser106) (OAEC00070)
Molecular Weight 66 kDa
NCBI Gene Id 2099
Host Rabbit
Clonality Polyclonal
Concentration 1 mg/ml
Gene Full Name Estrogen receptor
Alias Symbols ER, ESR, Era, ESRA, ESTRR, NR3A1
Product Format Liquid
Reference Medunjanin S, et al. (2005). J Biol Chem.80 (38):33006-33014. Dutertre M, et al. (2003). Mol Endocrinol.17 (7): 1296-1314. Chen D, et al. (2000). Mol Cell.6 (1): 127-137.
Reconstitution and Storage Store at -20C/1 year
Datasheets/Manuals Printable datasheet for anti-ESR1 antibody OAEC00070
Specificity Estrogen Receptor-? (phospho-Ser106) antibody detects endogenous levels of Estrogen Receptor-? only when phosphorylated at serine 106.
Application Info WB: 1:500-1:1000, IHC: 1:50-1:100, IF:1:100-1:200
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Estrogen Receptor-? around the phosphorylation site of serine106 (S-P- SP-P-L).
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Uniprot ID P03372
Protein Name Estrogen receptor
Protein Accession # NP_000116.2
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation
Gene Symbol ESR1
Predicted Species Reactivity Human, Mouse
Application WB, IHC, IF
Image 1
Human breast carcinoma
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-α (phospho-Ser106) antibody (OAEC00070)
Image 2
MCF-7
Western blot analysis of extracts from MCF-7 cells, using Estrogen Receptor-α (Ab-106) antibody (OAEC00416) and Estrogen Receptor-α (phospho-Ser106) antibody (OAEC00070
Image 3
MCF-7
Immunofluroescence staining of methanol-fixed MCF7 cells using ESR1 Antibody (OAEC00070, Red)
 

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