Product Number |
OAEC00070 |
Product Page |
www.avivasysbio.com/estrogen-receptor-antibody-phospho-ser106-oaec00070.html |
Name |
Estrogen Receptor-a Antibody (Phospho-Ser106) (OAEC00070) |
Molecular Weight |
66 kDa |
NCBI Gene Id |
2099 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1 mg/ml |
Gene Full Name |
Estrogen receptor |
Alias Symbols |
ER, ESR, Era, ESRA, ESTRR, NR3A1 |
Product Format |
Liquid |
Reference |
Medunjanin S, et al. (2005). J Biol Chem.80 (38):33006-33014. Dutertre M, et al. (2003). Mol Endocrinol.17 (7): 1296-1314. Chen D, et al. (2000). Mol Cell.6 (1): 127-137. |
Reconstitution and Storage |
Store at -20C/1 year |
Datasheets/Manuals |
Printable datasheet for anti-ESR1 antibody OAEC00070 |
Specificity |
Estrogen Receptor-? (phospho-Ser106) antibody detects endogenous levels of Estrogen Receptor-? only when phosphorylated at serine 106. |
Application Info |
WB: 1:500-1:1000, IHC: 1:50-1:100, IF:1:100-1:200 |
Immunogen |
The antiserum was produced against synthesized phosphopeptide derived from human Estrogen Receptor-? around the phosphorylation site of serine106 (S-P- SP-P-L). |
Formulation |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Uniprot ID |
P03372 |
Protein Name |
Estrogen receptor |
Protein Accession # |
NP_000116.2 |
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation |
Gene Symbol |
ESR1 |
Predicted Species Reactivity |
Human, Mouse |
Application |
WB, IHC, IF |
Image 1 | Human breast carcinoma
| Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-α (phospho-Ser106) antibody (OAEC00070) |
| Image 2 | MCF-7
| Western blot analysis of extracts from MCF-7 cells, using Estrogen Receptor-α (Ab-106) antibody (OAEC00416) and Estrogen Receptor-α (phospho-Ser106) antibody (OAEC00070 |
| Image 3 | MCF-7
| Immunofluroescence staining of methanol-fixed MCF7 cells using ESR1 Antibody (OAEC00070, Red) |
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