Akt Antibody (Phospho-Thr308) (OAEC00054)

Data Sheet
 
Product Number OAEC00054
Product Page www.avivasysbio.com/akt-antibody-phospho-thr308-oaec00054.html
Name Akt Antibody (Phospho-Thr308) (OAEC00054)
Isotype IgG
NCBI Gene Id 207
Host Rabbit
Clonality Polyclonal
Concentration 1 mg/ml
Gene Full Name RAC-alpha serine/threonine-protein kinase
Alias Symbols AKT, PKB, RAC, PRKBA, PKB-ALPHA, RAC-ALPHA
Product Format Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Reference Tremblay F, et al. (2005)Diabetes; 54(9): 2674-84. Xu BE, et al. (2005)J Biol Chem; 280(40): 34218-23. Samuels Y, et al. (2005)Cancer Cell; 7(6): 561-73. Di Maira G, et al. (2005)Cell Death Differ; 12(6): 668-77.
Reconstitution and Storage Store at -20C/1 year
Datasheets/Manuals Printable datasheet for anti-AKT1 antibody OAEC00054
Specificity Akt (phospho-Thr308) antibody detects endogenous levels of Akt only when phosphorylated at threonine 308.
Application Info WB: 1:500~1000
IHC: 1:50~100
IF: 1:100~200
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of threonine 308 (M-K-TP-F-C).
Intended Use For research use only.
Uniprot ID P31749
Protein Name RAC-alpha serine/threonine-protein kinase
Protein Accession # NP_001014431.1
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Gene Symbol AKT1
Predicted Species Reactivity Human, Mouse, Rat
Application WB, IHC, IF
Image 1
HeLa
Immunofluroescence staining of methanol-fixed HeLa cells showing nuclear dot staining using AKT1 Antibody (OAEC00054)
Image 2
293
Western blot analysis using Akt (phospho-Thr308) antibody (OAEC00054): Lane1: The extract from 293 cells untreated; Lane 2: The extract from 293 cells treated with insulin.
Image 3
Human lung carcinoma
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (phospho-Thr308) antibody(OAEC00054).
 

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