Product Number |
OAEC00054 |
Product Page |
www.avivasysbio.com/akt-antibody-phospho-thr308-oaec00054.html |
Name |
Akt Antibody (Phospho-Thr308) (OAEC00054) |
Isotype |
IgG |
NCBI Gene Id |
207 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1 mg/ml |
Gene Full Name |
RAC-alpha serine/threonine-protein kinase |
Alias Symbols |
AKT, PKB, RAC, PRKBA, PKB-ALPHA, RAC-ALPHA |
Product Format |
Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Reference |
Tremblay F, et al. (2005)Diabetes; 54(9): 2674-84. Xu BE, et al. (2005)J Biol Chem; 280(40): 34218-23. Samuels Y, et al. (2005)Cancer Cell; 7(6): 561-73. Di Maira G, et al. (2005)Cell Death Differ; 12(6): 668-77. |
Reconstitution and Storage |
Store at -20C/1 year |
Datasheets/Manuals |
Printable datasheet for anti-AKT1 antibody OAEC00054 |
Specificity |
Akt (phospho-Thr308) antibody detects endogenous levels of Akt only when phosphorylated at threonine 308. |
Application Info |
WB: 1:500~1000 IHC: 1:50~100 IF: 1:100~200
|
Immunogen |
The antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of threonine 308 (M-K-TP-F-C). |
Intended Use |
For research use only. |
Uniprot ID |
P31749 |
Protein Name |
RAC-alpha serine/threonine-protein kinase |
Protein Accession # |
NP_001014431.1 |
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site. |
Gene Symbol |
AKT1 |
Predicted Species Reactivity |
Human, Mouse, Rat |
Application |
WB, IHC, IF |
Image 1 | HeLa
| Immunofluroescence staining of methanol-fixed HeLa cells showing nuclear dot staining using AKT1 Antibody (OAEC00054) |
| Image 2 | 293
| Western blot analysis using Akt (phospho-Thr308) antibody (OAEC00054): Lane1: The extract from 293 cells untreated; Lane 2: The extract from 293 cells treated with insulin. |
| Image 3 | Human lung carcinoma
| Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (phospho-Thr308) antibody(OAEC00054). |
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