STAT3 Antibody (Phospho-Ser727) (OAEC00045)

• Product Number: OAEC00045
• Name: STAT3 Antibody (Phospho-Ser727) (OAEC00045)
• Molecular Weight: 88 kDa
• NCBI Gene Id: 6774
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Gene Full Name: Signal transducer and activator of transcription 3
• Alias Symbols: APRF, HIES, ADMIO, ADMIO1
• Product Format: Liquid
• Reference: Heim M H, (1999) J Recept Signal Transduct Res. 19: 75-120. Takeda K, et al. (1997) Proc Natl Acad Sci USA. 94: 3801-3804. Catlett-Falcone R, et al. (1999) Immunity. 10: 105-115.
• Reconstitution and Storage: Store at -20C/1 year
• Specificity: STAT3 (phospho-Ser727) antibody detects endogenous levels of STAT3 only when phosphorylated at serine 727.
• Application Info: WB: 1:500-1:1000, IHC: 1:50-1:100, IF:1:100-1:200
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human STAT3 around the phosphorylation site of serine 727 (P-M-S^P-P-R).
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Uniprot ID: P40763
• Protein Name: Signal transducer and activator of transcription 3
• Protein Accession #: NP_003141.2
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation
• Gene Symbol: STAT3
• Predicted Species Reactivity: Human, Mouse, Rat
• Application: WB, IHC, IF
• Image 1: Human breast carcinoma
  
  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT3 (phospho-Ser727) antibody (OAEC00045).
• Image 2: Human Hela
  
  Western blot analysis of extracts from HeLa cells using STAT3 (phospho-Ser727) antibody (OAEC00045
• Image 3: HeLa
  
  Immunofluroescence staining of methanol-fixed HeLa cells using STAT3 Antibody (OAEC00045, Red)