Elk-1 Antibody (Phospho-Thr417) (OAEC00038)

• Product Number: OAEC00038
• Name: Elk-1 Antibody (Phospho-Thr417) (OAEC00038)
• Molecular Weight: 45 kDa
• NCBI Gene Id: 2002
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Gene Full Name: ETS domain-containing protein Elk-1
• Product Format: Liquid
• Reference: Huang Y, et al. (2000) Biochem Biophys Res Commun. 271(2): 456-463. Marais R, et al. (1993) Cell 73:381-393. Kortenjann M, et al. (1994) Mol Cell Biol. 14:4815-4824. Hill C S, et al. (1995) Cell. 80:199-211. Cavigelli M, et al. (1995) EMBO J. 14:5957-5964
• Reconstitution and Storage: Store at -20C/1 year
• Specificity: Elk-1 (phospho-Thr417) antibody detects endogenous levels of Elk-1 only when phosphorylated at threonine 417.
• Application Info: IHC: 1:50-1:100, IF:1:100-1:200
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Elk-1 around the phosphorylation site of threonine 417 (L-S-T^P-P-V).
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Uniprot ID: P19419
• Protein Name: ETS domain-containing protein Elk-1
• Protein Accession #: NP_001107595.1
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation
• Gene Symbol: ELK1
• Predicted Species Reactivity: Human, Mouse, Rat
• Application: IHC, IF
• Image 1: Human breast carcinoma
  
  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk-1 (phospho-Thr417) antibody (OAEC00038).
• Image 2: Human Hela
  
  Immunofluorescence staining of methanol-fixed HeLa cells using Elk-1 (phospho-Thr417) antibody (OAEC00038, Red).