MEK2 Antibody (Phospho-Thr394) (OAEC00008)

• Product Number: OAEC00008
• Name: MEK2 Antibody (Phospho-Thr394) (OAEC00008)
• Molecular Weight: 44 kDa
• NCBI Gene Id: 5605
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Gene Full Name: Dual specificity mitogen-activated protein kinase kinase 2
• Alias Symbols: CFC4, MEK2, MKK2, MAPKK2, PRKMK2
• Product Format: Liquid
• Reference: Crews C M, et al. (1992) Science. 258:478-480. Alessi R, et al. (1994) EMBO J. 13:1610-1619. Rosen L B, et al. (1994) Neuron. 12:1207-1221.
• Reconstitution and Storage: Store at -20C/1 year
• Specificity: MEK2 (phospho-Thr394) antibody detects endogenous evels of MEK2 only when phosphorylated at threonine 94.
• Application Info: WB: 1:500-1:1000, IHC: 1:50-1:100, IF: 1:100-200
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human MEK2 around the phosphorylation site of threonine 394 (P-G-T^P-P-T).
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Uniprot ID: P36507
• Protein Name: Dual specificity mitogen-activated protein kinase kinase 2
• Protein Accession #: NP_109587.1
• Purification: The antibody was affinity-purified from rabbit antiserum y affinity-chromatography using epitope-specific hosphopeptide. The antibody against non- hosphopeptide was removed by chromatogramphy sing non-phosphopeptide corresponding to the hosphorylation sit
• Gene Symbol: MAP2K2
• Predicted Species Reactivity: Human, Mouse, Rat
• Application: WB, IHC, IF
• Image 1: HeLa
  
  Immunofluroescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using MAP2K2 Antibody (OAEC00008)
• Image 2: Human breast carcinoma
  
  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK2 (phospho-Thr394) antibody (OAEC00008).
• Image 3: ovary cancer
  
  Western blot analysis of extracts from ovary cancer cells using MEK2 (phospho-Thr394) antibody (OAEC00008).