Product Number |
OAEC00007 |
Product Page |
www.avivasysbio.com/gsk3-antibody-phospho-ser21-oaec00007.html |
Name |
GSK3a Antibody (Phospho-Ser21) (OAEC00007) |
Molecular Weight |
51 kDa |
NCBI Gene Id |
2931 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1 mg/ml |
Gene Full Name |
Glycogen synthase kinase-3 alpha |
Product Format |
Liquid |
Reference |
Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178. Koivisto L, et al. (2003) J Cell Sci. 116(Pt 18): 3749-3760. Welsh G I, et al. (1996) Trends Cell Biol. 6:274-279. Srivastava A K, et al. (1998) Mol. Cell. Biochem. 182: 135-141. |
Reconstitution and Storage |
Store at -20C/1 year |
Datasheets/Manuals |
Printable datasheet for anti-GSK3A antibody OAEC00007 |
Specificity |
GSK3? (phospho-Ser21) antibody detects endogenous levels of GSK3? only when phosphorylated at serine 21. |
Application Info |
WB: 1:500-1:1000, IHC: 1:50-1:100, IF: 1:100-200 |
Immunogen |
The antiserum was produced against synthesized phosphopeptide derived from human GSK3? around the phosphorylation site of serine 21 (T-S-SP-F-A). |
Formulation |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Uniprot ID |
P49840 |
Protein Name |
Glycogen synthase kinase-3 alpha |
Protein Accession # |
NP_063937.2 |
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation |
Gene Symbol |
GSK3A |
Predicted Species Reactivity |
Human, Mouse, Rat |
Application |
WB, IHC, IF |
Image 1 | Human breast carcinoma
| Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3α (phospho-Ser21) antibody (OAEC00007). |
| Image 2 | ovary cancer
| Western blot analysis of extracts from ovary cancer cells using GSK3α (phospho-Ser21) antibody (OAEC00007 |
| Image 3 | HeLa
| Immunofluroescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using GSK3A Antibody (OAEC00007) |
|
|