GSK3a Antibody (Phospho-Ser21) (OAEC00007)

Data Sheet
 
Product Number OAEC00007
Product Page www.avivasysbio.com/gsk3-antibody-phospho-ser21-oaec00007.html
Name GSK3a Antibody (Phospho-Ser21) (OAEC00007)
Molecular Weight 51 kDa
NCBI Gene Id 2931
Host Rabbit
Clonality Polyclonal
Concentration 1 mg/ml
Gene Full Name Glycogen synthase kinase-3 alpha
Product Format Liquid
Reference Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178. Koivisto L, et al. (2003) J Cell Sci. 116(Pt 18): 3749-3760. Welsh G I, et al. (1996) Trends Cell Biol. 6:274-279. Srivastava A K, et al. (1998) Mol. Cell. Biochem. 182: 135-141.
Reconstitution and Storage Store at -20C/1 year
Datasheets/Manuals Printable datasheet for anti-GSK3A antibody OAEC00007
Specificity GSK3? (phospho-Ser21) antibody detects endogenous levels of GSK3? only when phosphorylated at serine 21.
Application Info WB: 1:500-1:1000, IHC: 1:50-1:100, IF: 1:100-200
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human GSK3? around the phosphorylation site of serine 21 (T-S-SP-F-A).
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Uniprot ID P49840
Protein Name Glycogen synthase kinase-3 alpha
Protein Accession # NP_063937.2
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation
Gene Symbol GSK3A
Predicted Species Reactivity Human, Mouse, Rat
Application WB, IHC, IF
Image 1
Human breast carcinoma
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3α (phospho-Ser21) antibody (OAEC00007).
Image 2
ovary cancer
Western blot analysis of extracts from ovary cancer cells using GSK3α (phospho-Ser21) antibody (OAEC00007
Image 3
HeLa
Immunofluroescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using GSK3A Antibody (OAEC00007)
 

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