PDK1 Antibody (Phospho-Ser241) (OAEC00005)

Data Sheet
 
Product Number OAEC00005
Product Page www.avivasysbio.com/pdk1-antibody-phospho-ser241-oaec00005.html
Name PDK1 Antibody (Phospho-Ser241) (OAEC00005)
Molecular Weight 63 kDa
Isotype IgG
NCBI Gene Id 5170
Host Rabbit
Clonality Polyclonal
Concentration 1 mg/ml
Gene Full Name 3-phosphoinositide-dependent protein kinase 1
Alias Symbols PDK1, PDPK2, PDPK2P, PRO0461
Product Format Liquid
Reference Scheid MP,et al. (2005)Mol Cell Biol; 25(6): 2347-63
Chen H, et al. (2001) Biochemistry; 40(39): 11851-9
Sato ,et al. (2002) J Biol Chem; 277(42): 39360-7
Lim MA, et al.(2003)Proc Natl Acad Sci U S A; 100(24): 14006-11
Reconstitution and Storage Store at -20C/1 year
Datasheets/Manuals Printable datasheet for anti-PDPK1 antibody OAEC00005
Specificity PDK1 (phospho-Ser241) antibody detects endogenous levels of PDK1 only when phosphorylated at serine 241.
Application Info WB: 1:500-1:1000, IHC: 1:50-1:100
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PDK1 around the phosphorylation site of serine 241 (A-N-SP-F-V).
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Uniprot ID O15530
Protein Name 3-phosphoinositide-dependent protein kinase 1
Protein Accession # NP_001248745.1
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Gene Symbol PDPK1
Predicted Species Reactivity Human, Mouse, Rat
Application WB, IHC
Image 1
Human breast carcinoma
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PDK1 (phospho-Ser241) antibody (OAEC00005).
Image 2
MDA-MB-435
Western blot analysis of extracts from MDA-MB-435 cells using PDK1 (phospho-Ser241) antibody (OAEC00005)
 

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