Product Number |
OAEC00005 |
Product Page |
www.avivasysbio.com/pdk1-antibody-phospho-ser241-oaec00005.html |
Name |
PDK1 Antibody (Phospho-Ser241) (OAEC00005) |
Molecular Weight |
63 kDa |
Isotype |
IgG |
NCBI Gene Id |
5170 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1 mg/ml |
Gene Full Name |
3-phosphoinositide-dependent protein kinase 1 |
Alias Symbols |
PDK1, PDPK2, PDPK2P, PRO0461 |
Product Format |
Liquid |
Reference |
Scheid MP,et al. (2005)Mol Cell Biol; 25(6): 2347-63 Chen H, et al. (2001) Biochemistry; 40(39): 11851-9 Sato ,et al. (2002) J Biol Chem; 277(42): 39360-7 Lim MA, et al.(2003)Proc Natl Acad Sci U S A; 100(24): 14006-11 |
Reconstitution and Storage |
Store at -20C/1 year |
Datasheets/Manuals |
Printable datasheet for anti-PDPK1 antibody OAEC00005 |
Specificity |
PDK1 (phospho-Ser241) antibody detects endogenous levels of PDK1 only when phosphorylated at serine 241. |
Application Info |
WB: 1:500-1:1000, IHC: 1:50-1:100 |
Immunogen |
The antiserum was produced against synthesized phosphopeptide derived from human PDK1 around the phosphorylation site of serine 241 (A-N-SP-F-V). |
Formulation |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Uniprot ID |
O15530 |
Protein Name |
3-phosphoinositide-dependent protein kinase 1 |
Protein Accession # |
NP_001248745.1 |
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site. |
Gene Symbol |
PDPK1 |
Predicted Species Reactivity |
Human, Mouse, Rat |
Application |
WB, IHC |
Image 1 | Human breast carcinoma
| Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PDK1 (phospho-Ser241) antibody (OAEC00005). |
| Image 2 | MDA-MB-435
| Western blot analysis of extracts from MDA-MB-435 cells using PDK1 (phospho-Ser241) antibody (OAEC00005) |
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