| Product Number |
OAEB02508 |
| Product Page |
www.avivasysbio.com/stat4-antibody-oaeb02508.html |
| Name |
STAT4 Antibody (OAEB02508) |
| NCBI Gene Id |
6775 |
| Host |
Goat |
| Clonality |
Polyclonal |
| Purity |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Alias Symbols |
SLEB11 |
| Peptide Sequence |
C-DHSESGEVRFHSVEP |
| Product Format |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Reference |
* Good SR, Thieu VT, Mathur AN, Yu Q, Stritesky GL, Yeh N, O'Malley JT, Perumal NB, Kaplan MH,; Temporal induction pattern of STAT4 target genes defines potential for Th1 lineage-specific programming.; Journal of immunology (Baltimore, Md. : 1950) 2009 Sep 183 (6): 3839-47.; Pubmed 19710469 |
| Reconstitution and Storage |
Aliquot and store at -20C. Minimize freezing and thawing. |
| Datasheets/Manuals |
Printable datasheet for anti-STAT4 antibody - OAEB02508 |
| Additional Information |
Reactivity Info: Tested: Human; Expected: Human, Dog, Pig |
| Application Info |
Peptide ELISA: antibody detection limit dilution 1:32000.
Western Blot: In transfected HEK293 transiently expressing full-length Human STAT4 (MYC and DYKDDDDK tagged), a band of approx.85kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.2-0.5ug/ml. |
| Uniprot ID |
Q14765 |
| Protein Name |
Signal transducer and activator of transcription 4 |
| Protein Accession # |
NP_003142.1 |
| Gene Symbol |
STAT4 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit |
| Application |
WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 93%; Dog: 93%; Horse: 93%; Human: 100%; Mouse: 86%; Pig: 93%; Rabbit: 93%; Rat: 86% |
| Image 1 | HEK293
 | HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human STAT4 (RC206892) with C-terminal MYC tag probed with OAEB02508 (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with OAEB02508 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence. |
|
|
