RARA Antibody (OAEB02477)

Data Sheet
 
Product Number OAEB02477
Product Page www.avivasysbio.com/rara-antibody-oaeb02477.html
Name RARA Antibody (OAEB02477)
Molecular Weight 50.8; 50.8; 39.7
NCBI Gene Id 5914
Host Goat
Clonality Polyclonal
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration 0.5 mg/ml
Gene Full Name retinoic acid receptor alpha
Alias Symbols NR1B1;nuclear receptor subfamily 1 group B member 1;nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form;PML-DDX5-RARA fusion;PML-DDX5-RARA fusion protein;RAR;RAR-alpha;retinoic acid nuclear receptor alpha variant 1;retinoic acid nuclear receptor alpha variant 2;retinoic acid receptor alpha;retinoic acid receptor, alpha polypeptide.
Peptide Sequence C-SPSLSPSSHRSSPATQSP
Product Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reconstitution and Storage Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals Printable datasheet for RARA Antibody (OAEB02477)
Additional Information Reactivity Info: Tested: Human; Expected: Human, Mouse, Rat, Dog, Cow
Application Info Peptide ELISA: antibody detection limit dilution 1:8000.
Western Blot: Approx 50kDa band observed in Human Brain (Cerebellum and Hippocampus) lysates (calculated MW of 50.8kDa according to NP_000955.1; NP_001019980.1). Recommended concentration: 1-3ug/ml.
Immunohistochemistry: In paraffin embedded Human Cerebellum shows staining of Purkinje cytoplasm. Recommended concentration, 4-6ug/ml.
Immunogen RARA
Uniprot ID P10276
Protein Name Retinoic acid receptor alpha
Protein Accession # NP_000955.1
Purification Ammonium Sulfate Precipitated; Affinity Purified
Gene Symbol RARA
Predicted Species Reactivity Bovine|Dog|Human|Mouse|Rat
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Cow: 88%; Dog: 88%; Human: 93%; Mouse: 100%; Rabbit: 88%; Rat: 100%; Zebrafish: 77%
Image 1
Human Cerebellum
OAEB02477 (4ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Image 2
Human Cerebellum
OAEB02477 (0.05ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 

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