Goat Anti-ABCC4 / MRP4 Antibody (OAEB02455)

• Product Number: OAEB02455
• Name: Goat Anti-ABCC4 / MRP4 Antibody (OAEB02455)
• Molecular Weight: 150
• NCBI Gene Id: 10257|170924
• Host: Goat
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: ATP binding cassette subfamily C member 4
• Alias Symbols: ABC-tranporter;ATP-binding cassette protein C4;ATP-binding cassette subfamily C member 4;ATP-binding cassette sub-family C member 4;ATP-binding cassette, subfamily C (CFTR/MRP), member 4;ATP-binding cassette, sub-family C (CFTR/MRP), member 4;bA464I2.1 (ATP-binding cassette, sub-family C (CFTR/MRP), member 4);canalicular multispecific organic anion transporter (ABC superfamily);MOATB;MOAT-B;MRP/cMOAT-related ABC transporter;Mrp4;multidrug resistance-associated protein 4;multi-specific organic anion transporter B;RGD1565953.
• Peptide Sequence: CNGQPSTLTIFETAL
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Reference: * Schuetz JD, Connelly MC, Sun D, Paibir SG, Flynn PM, Srinivas RV, Kumar A, Fridland A.; MRP4: A previously unidentified factor in resistance to nucleoside-based antiviral drugs.; Nat Med. 1999 Sep;5(9):1048-51.; Pubmed 10470083
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Specificity: This antibody is expected to recognize isoform 1 (NP_005836.2) only.
• Application Info: P-ELISA 1:32000
  WB 1.0~3.0 ug/ml, 170 kDa band observed in rat renal cortex membranes.
  IHC 2.0~4.0 ug/ml. Shows strong cytoplasmic staining in small flat basal cells and weak cytoplasmic staining in the columnar secretory cells of the epithelium.
  IF Used successfully in human primary hepatocytes as describied in Xu et al, Am J Physiol Gastrointest Liver Physiol. 2010 Jul;200(1):G126-35, PMID: 20395535.
• Immunogen: ABCC4
• Uniprot ID: O15439
• Protein Name: Multidrug resistance-associated protein 4
• Publications:

  Gori, I. et al. Augmented epithelial multidrug resistance-associated protein 4 expression in peritoneal endometriosis: regulation by lipoxin A(4). Fertil. Steril. 99, 1965-73.e2 (2013). 23472950

  Montani, M. et al. Multidrug resistance protein 4 (MRP4) expression in prostate cancer is associated with androgen signaling and decreases with tumor progression. Virchows Arch. 462, 437-43 (2013). 23503867

  Weerachayaphorn, J. et al. Nuclear factor-E2-related factor 2 is a major determinant of bile acid homeostasis in the liver and intestine. Am. J. Physiol. Gastrointest. Liver Physiol. 302, G925-36 (2012). 22345550

  Xu, S., Weerachayaphorn, J., Cai, S.-Y., Soroka, C. J. & Boyer, J. L. Aryl hydrocarbon receptor and NF-E2-related factor 2 are key regulators of human MRP4 expression. Am. J. Physiol. Gastrointest. Liver Physiol. 299, G126-35 (2010). 20395535

• Protein Accession #: NP_005836.2
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Gene Symbol: Abcc4|ABCC4
• Predicted Species Reactivity: Human|Mouse|Rat
• Application: IHC-P, P-WB, IF
• Predicted Homology Based on Immunogen Sequence: Human, Mouse, Rat
• Image 1: Human Prostate
  
  OAEB02455 (4ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.