SOCS1 Antibody (OAEB02451)

Data Sheet
 
Product Number OAEB02451
Product Page www.avivasysbio.com/socs1-antibody-oaeb02451.html
Name SOCS1 Antibody (OAEB02451)
Molecular Weight 23.6
NCBI Gene Id 12703|8651
Host Goat
Clonality Polyclonal
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration 0.5 mg/ml
Gene Full Name suppressor of cytokine signaling 1
Alias Symbols AISIMD;Cis;CIS1;Cish1;Cish7;cytokine inducible SH2-containing protein 1;cytokine inducible SH2-containing protein 7;cytokine-inducible SH2 protein 1;JA;JAB;JAK binding protein;JAK2-binding protein;SOC;SOCS-1;SS;SSI1;SSI-1;STAT induced SH3 protein 1;STAT-induced STAT inhibitor 1;suppressor of cytokine signaling 1;Tec-interacting protein 3;TIP3;TIP-3.
Peptide Sequence C-VLRDYLSSFPFQI
Product Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reference * Starr R, Willson TA, Viney EM, Murray LJ, Rayner JR, Jenkins BJ, Gonda TJ, Alexander WS, Metcalf D, Nicola NA, Hilton DJ.; A family of cytokine-inducible inhibitors of signalling.; Nature 1997 Jun 26;387(6636):917-21; Pubmed 9202125
Reconstitution and Storage Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals Printable datasheet for SOCS1 Antibody (OAEB02451)
Application Info Peptide ELISA: antibody detection limit dilution 1:4000.
Western Blot: In transfected HEK293 transiently expressing human SOCS1 bands of approx. 30kDa and 25kDa are observed. These bands are not observed in the non-transfected HEK293. A similar pattern is observed in Mouse Spleen lysates. The calculated molecular size is 23.6kDa according to Human NP_003736.1 and 23.7kDa according to Mouse NP_034026.1. Recommended concentration, 1-3ug/ml.
Immunohistochemistry: In paraffin embedded Human Kidney shows strong staining of the Brush Border in PCT. Recommended concentration, 2-3ug/ml.
Immunogen SOCS1
Uniprot ID O15524
Protein Name Suppressor of cytokine signaling 1
Protein Accession # NP_003736.1
Purification Ammonium Sulfate Precipitated; Affinity Purified
Gene Symbol Socs1|SOCS1
Predicted Species Reactivity Bovine|Dog|Human|Mouse|Porcine|Rat
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%
Image 1
HEK293
HEK293 overexpressing SOCS1 and probed with OAEB02451 (mock transfection in lane B).
Image 2
Human Kidney
OAEB02451 (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Image 3
mouse spleen
OAEB02451 staining (2ug/ml) of mouse spleen lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
 

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