Product Number |
OAEB02451 |
Product Page |
www.avivasysbio.com/socs1-antibody-oaeb02451.html |
Name |
SOCS1 Antibody (OAEB02451) |
Molecular Weight |
23.6 |
NCBI Gene Id |
12703|8651 |
Host |
Goat |
Clonality |
Polyclonal |
Purity |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Concentration |
0.5 mg/ml |
Gene Full Name |
suppressor of cytokine signaling 1 |
Alias Symbols |
AISIMD;Cis;CIS1;Cish1;Cish7;cytokine inducible SH2-containing protein 1;cytokine inducible SH2-containing protein 7;cytokine-inducible SH2 protein 1;JA;JAB;JAK binding protein;JAK2-binding protein;SOC;SOCS-1;SS;SSI1;SSI-1;STAT induced SH3 protein 1;STAT-induced STAT inhibitor 1;suppressor of cytokine signaling 1;Tec-interacting protein 3;TIP3;TIP-3. |
Peptide Sequence |
C-VLRDYLSSFPFQI |
Product Format |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Reference |
* Starr R, Willson TA, Viney EM, Murray LJ, Rayner JR, Jenkins BJ, Gonda TJ, Alexander WS, Metcalf D, Nicola NA, Hilton DJ.; A family of cytokine-inducible inhibitors of signalling.; Nature 1997 Jun 26;387(6636):917-21; Pubmed 9202125 |
Reconstitution and Storage |
Aliquot and store at -20C. Minimize freezing and thawing. |
Datasheets/Manuals |
Printable datasheet for SOCS1 Antibody (OAEB02451) |
Application Info |
Peptide ELISA: antibody detection limit dilution 1:4000. Western Blot: In transfected HEK293 transiently expressing human SOCS1 bands of approx. 30kDa and 25kDa are observed. These bands are not observed in the non-transfected HEK293. A similar pattern is observed in Mouse Spleen lysates. The calculated molecular size is 23.6kDa according to Human NP_003736.1 and 23.7kDa according to Mouse NP_034026.1. Recommended concentration, 1-3ug/ml. Immunohistochemistry: In paraffin embedded Human Kidney shows strong staining of the Brush Border in PCT. Recommended concentration, 2-3ug/ml. |
Immunogen |
SOCS1 |
Uniprot ID |
O15524 |
Protein Name |
Suppressor of cytokine signaling 1 |
Protein Accession # |
NP_003736.1 |
Purification |
Ammonium Sulfate Precipitated; Affinity Purified |
Gene Symbol |
Socs1|SOCS1 |
Predicted Species Reactivity |
Bovine|Dog|Human|Mouse|Porcine|Rat |
Application |
WB, IHC |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100% |
Image 1 | HEK293
| HEK293 overexpressing SOCS1 and probed with OAEB02451 (mock transfection in lane B). |
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Image 2 | Human Kidney
| OAEB02451 (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
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Image 3 | mouse spleen
| OAEB02451 staining (2ug/ml) of mouse spleen lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
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