Goat Anti-ENPP1 / PC1 Antibody (OAEB02445)

Data Sheet
 
Product Number OAEB02445
Product Page www.avivasysbio.com/goat-anti-enpp1-pc1-antibody-oaeb02445.html
Name Goat Anti-ENPP1 / PC1 Antibody (OAEB02445)
Molecular Weight 105
NCBI Gene Id 5167
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name ectonucleotide pyrophosphatase/phosphodiesterase 1
Alias Symbols alkaline phosphodiesterase 1;ARHR2;COLED;ectonucleotide pyrophosphatase/phosphodiesterase family member 1;E-NPP 1;Ly-41 antigen;M6S1;membrane component, chromosome 6, surface marker 1;NPP1;NPPS;PC-1;PCA1;PDNP1;phosphodiesterase I/nucleotide pyrophosphatase 1;plasma-cell membrane glycoprotein 1;plasma-cell membrane glycoprotein PC-1.
Peptide Sequence C-KTHLPTFSQED
Product Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reference * Orriss IR, Utting JC, Brandao-Burch A, Colston K, Grubb BR, Burnstock G, Arnett TR.; Extracellular nucleotides block bone mineralization in vitro: evidence for dual inhibitory mechanisms involving both P2Y2 receptors and pyrophosphate.; Endocrinology. 2007 Sep;148(9):4208-16.; Pubmed 17569759
* Meyre D, Bouatia-Naji N, Tounian A, Samson C, Lecoeur C, Vatin V, Ghoussaini M, Wachter C, Hercberg S, Charpentier G, Patsch W, Pattou F, Charles MA, Tounian P, Clement K, Jouret B, Weill J, Maddux BA, Goldfine ID, Walley A, Boutin P, Dina C, Froguel P.; Variants of ENPP1 are associated with childhood and adult obesity and increase the risk of glucose intolerance and type 2 diabetes.; Nat Genet. 2005 Aug;37(8):863-7. Epub 2005 Jul 17.; Pubmed 16025115
Reconstitution and Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Datasheets/Manuals Printable datasheet for Goat Anti-ENPP1 / PC1 Antibody (OAEB02445)
Application Info P-ELISA: Antibody detection limit dilution 1:32000
IHC: Paraffin embedded Human Thyroid Gland. Recommended concentration: 8 ug/mL.
Immunogen ENPP1
Uniprot ID P22413
Protein Name Ectonucleotide pyrophosphatase/phosphodiesterase family member 1
Publications

de la Croix Ndong, J. et al. Asfotase-a improves bone growth, mineralization and strength in mouse models of neurofibromatosis type-1. Nat. Med. 20, 904-10 (2014). 24997609

Protein Accession # NP_006199.2
Purification Ammonium Sulfate Precipitated; Affinity Purified
Application Data This antibody has been successfully used in Western blot and IHC:
Lau WM, Doucet M, Stadel R, Huang D, Weber KL, Kominsky SL
Enpp1: a potential facilitator of breast cancer bone metastasis.
PLoS One. 2013 Jul 5;8(7):e66752.
PMID: 23861746
This antibody has been successfully used in Western blot:
Simão AM, Yadav MC, Narisawa S, Bolean M, Pizauro JM, Hoylaerts MF, Ciancaglini P, Millán JL.
Proteoliposomes harboring alkaline phosphatase and nucleotide pyrophosphatase as matrix vesicle biomimetics.
J Biol Chem. 2010 Mar 5;285(10):7598-609.
PMID: 20048161.
Tested Species Reactivity Human
Gene Symbol ENPP1
Predicted Species Reactivity Human|Mouse|Rat
Application IHC, P-ELISA, WB
Image 1
HEK293
HEK293 overexpressing Human ENPP1 (RC209222) and probed with OAEB02445 (mock transfection in first lane), tested by Origene.
Image 2
Human Liver
OAEB02445 (1ug/ml) staining of paraffin embedded Human Liver. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Image 3
Immunohistochemistry
(8ug/ml) staining of paraffin embedded Human Thyroid Gland. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Image 4
Immunohistochemistry
Negative Control showing staining of paraffin embedded Human Thyroid Gland, with no primary antibody.
 

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