Goat Anti-CENTB2 / ACAP2 Antibody (OAEB02433)

Data Sheet
 
Product Number OAEB02433
Product Page www.avivasysbio.com/goat-anti-centb2-acap2-antibody-oaeb02433.html
Name Goat Anti-CENTB2 / ACAP2 Antibody (OAEB02433)
Molecular Weight 88.1
NCBI Gene Id 23527|78618
Host Goat
Clonality Polyclonal
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration 0.5 mg/ml
Gene Full Name ArfGAP with coiled-coil, ankyrin repeat and PH domains 2
Alias Symbols 4832442G16;9530039J15Rik;Arf GAP with coiled coil, ANK repeat and PH domains 2;arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2;centaurin, beta 2;centaurin-beta-2;Centb;Centb2;CNT-B2;mKIAA0041.
Peptide Sequence C-EKLNRFQQDSQKF
Product Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reference * Jackson TR, Brown FD, Nie Z, Miura K, Foroni L, Sun J, Hsu VW, Donaldson JG, Randazzo PA.; ACAPs are arf6 GTPase-activating proteins that function in the cell periphery.; J Cell Biol. 2000 Oct 30;151(3):627-38.; Pubmed 11062263
Reconstitution and Storage Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals Printable datasheet for Goat Anti-CENTB2 / ACAP2 Antibody (OAEB02433)
Application Info Peptide ELISA: antibody detection limit dilution 1:32000.
Western Blot: Approx 90kDa band observed in Human T cell line MOLT4 lysates (calculated MW of 88.1kDa according to NP_036419). Recommended concentration: 1-3ug/ml.
Immunohistochemistry: In paraffin embedded Human Lung shows strong staining of Type II pneumocytes. Recommended concentration, 5-10ug/ml.
Immunogen ACAP2
Uniprot ID Q15057
Protein Name Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2
Protein Accession # NP_036419.3
Purification Ammonium Sulfate Precipitated; Affinity Purified
Gene Symbol Acap2|ACAP2
Predicted Species Reactivity Dog|Human|Mouse|Porcine|Rat
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
Image 1
Human Lung
OAEB02433 (5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Image 2
MOLT4
OAEB02433 (1ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 

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