| Product Number |
OAEB02431 |
| Product Page |
www.avivasysbio.com/goat-anti-fzd8-frizzled-8-antibody-oaeb02431.html |
| Name |
Goat Anti-FZD8 / frizzled 8 Antibody (OAEB02431) |
| Molecular Weight |
73.3 |
| NCBI Gene Id |
14370|8325 |
| Host |
Goat |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
frizzled class receptor 8 |
| Alias Symbols |
frizzled 8, seven transmembrane spanning receptor;frizzled family receptor 8;frizzled homolog 8;frizzled-8;fz-8;Fz8;hFZ8. |
| Peptide Sequence |
C-SYPKQMPLSQV |
| Product Format |
Liquid Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Reference |
* Keay SK, Szekely Z, Conrads TP, Veenstra TD, Barchi JJ Jr, Zhang CO, Koch KR, Michejda CJ.; An antiproliferative factor from interstitial cystitis patients is a frizzled 8 protein-related sialoglycopeptide.; Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11803-8. Epub 2004 Jul 28.; Pubmed 15282374 |
| Reconstitution and Storage |
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Datasheets/Manuals |
Printable datasheet for Goat Anti-FZD8 / frizzled 8 Antibody (OAEB02431) |
| Application Info |
P-ELISA: Antibody detection limit dilution 1:32000
WB: Approx 75kDa band observed in human heart and human pancreas lysates (calculated MW of 73.3kDa according to NP_114072.1). Recommended concentration: 1-3ug/ml.
IHC: In paraffin embedded Human Pancreas, shows granular cytoplasmic staining of acinar cells. Cells of the Islets of Langerhans are weakly stained, if at all. Recommended concentration, 3ug/ml.
IF: This antibody has been successfully used in IF on Mouse: Zhao B et al. (2019) PMID: 21321602. |
| Immunogen |
Peptide with sequence C-SYPKQMPLSQV, from the C Terminus of the protein sequence according to NP_114072.1. |
| Intended Use |
Research use only. Not for diagnostic or therapeutic use. |
| Uniprot ID |
Q9H461 |
| Protein Name |
Frizzled-8 |
| Publications |
Yin, S. et al. Tumor-initiating cells and FZD8 play a major role in drug resistance in triple-negative breast cancer. Mol. Cancer Ther. 12, 491-8 (2013). 23445611 |
| Protein Accession # |
NP_114072.1 |
| Purification |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Gene Symbol |
Fzd8|FZD8 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Dog, Cow |
| Application |
WB, IHC |
| Image 1 | Human Pancreas
 | OAEB02431 (3ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP- |
| | Image 2 | human pancreas
 | OAEB02431 (1ug/ml) staining of human pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
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