RANGAP1 Antibody (OAEB02428)

Data Sheet
Product Number OAEB02428
Product Page www.avivasysbio.com/rangap1-antibody-oaeb02428.html
Name RANGAP1 Antibody (OAEB02428)
Molecular Weight 63.5
NCBI Gene Id 19387|5905
Host Goat
Clonality Polyclonal
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration 0.5 mg/ml
Gene Full Name Ran GTPase activating protein 1|RAN GTPase activating protein 1
Alias Symbols C79654;Fug1;mKIAA1835;ran GTPase-activating protein 1;RANGAP;SD;segregation distorter homolog.
Product Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reference * Bischoff FR, Krebber H, Kempf T, Hermes I, Ponstingl H.; Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport.; Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1749-53.; Pubmed 7878053
Reconstitution and Storage Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals Printable datasheet for RANGAP1 Antibody (OAEB02428)
Application Info Peptide ELISA: antibody detection limit dilution 1:32000.
Western Blot: Approx 70kDa band observed in Human Brain and 3T3 lysates (predicted MW of 68kDa according to NP_002874). Recommended for use at 0.1-0.5ug/ml.
Immunohistochemistry: In paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatocytes. Recommended concentration, 2-4ug/ml.
Immunogen RANGAP1
Uniprot ID P46060
Protein Name Ran GTPase-activating protein 1
Protein Accession # NP_002874.1
Purification Ammonium Sulfate Precipitated; Affinity Purified
Gene Symbol Rangap1|RANGAP1
Predicted Species Reactivity Bovine|Dog|Human|Mouse|Rat
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
Image 1
OAEB02428 staining (0.2ug/ml) of 3T3 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Image 2
Human Liver
OAEB02428 (2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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