| Product Number |
OAEB02356 |
| Product Page |
www.avivasysbio.com/goat-anti-tcf3-itf1-antibody-oaeb02356.html |
| Name |
Goat Anti-TCF3 / ITF1 Antibody (OAEB02356) |
| Molecular Weight |
67.6 |
| NCBI Gene Id |
6929 |
| Host |
Goat |
| Clonality |
Polyclonal |
| Purity |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
transcription factor 3 |
| Alias Symbols |
AGM8;bHLHb21;class B basic helix-loop-helix protein 21;E2A;E2A-HLF fusion transcript protein;E47;helix-loop-helix protein HE47;immunoglobulin transcription factor 1;ITF1;kappa-E2-binding factor;negative vitamin D response element-binding protein;NOL1-TCF3 fusion;p75;TCF-3;transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47);transcription factor 3 variant 3;transcription factor E2-alpha;transcription factor ITF-1;VDIR;VDR interacting repressor;vitamin D receptor-interacting repressor. |
| Peptide Sequence |
C-KAPRARTSPDEDED |
| Product Format |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reference |
* Greenbaum S, Lazorchak AS, Zhuang Y.; Differential functions for the transcription factor E2A in positive and negative gene regulation in pre-B lymphocytes.; J Biol Chem. 2004 Oct 22;279(43):45028-35.; Pubmed 15310760 |
| Reconstitution and Storage |
Aliquot and store at -20C. Minimize freezing and thawing. |
| Datasheets/Manuals |
Printable datasheet for Goat Anti-TCF3 / ITF1 Antibody (OAEB02356) |
| Additional Information |
Reactivity Info: Tested: Human; Expected: Human |
| Application Info |
Peptide ELISA: antibody detection limit dilution 1:128000.
Western Blot: Approx 75kDa band observed in lysates of cell line Daudi (calculated MW of 67.6kDa according to NP_003191.1). Recommended concentration: 0.01-0.03ug/ml.
Immunohistochemistry: In paraffin embedded Human Kidney shows strong nuclear staining in DCT cells. Recommended concentration, 3-5ug/ml. |
| Immunogen |
TCF3 |
| Uniprot ID |
P15923 |
| Protein Name |
Transcription factor E2-alpha |
| Protein Accession # |
NP_003191.1 |
| Purification |
Ammonium Sulfate Precipitated; Affinity Purified |
| Gene Symbol |
TCF3 |
| Predicted Species Reactivity |
Human |
| Application |
WB, IHC |
| Predicted Homology Based on Immunogen Sequence |
Dog: 78%; Human: 100%; Mouse: 85%; Pig: 92%; Rat: 85% |
| Image 1 | Daudi
 | OAEB02356 (0.01ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
| | Image 2 | Human Kidney
 | OAEB02356 (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
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