DCTN1 Antibody (OAEB02346)

Data Sheet
 
Product Number OAEB02346
Product Page www.avivasysbio.com/dctn1-antibody-oaeb02346.html
Name DCTN1 Antibody (OAEB02346)
Molecular Weight 142; 127; 139; 127, 136, 140
NCBI Gene Id 13191|1639
Host Goat
Clonality Polyclonal
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration 0.5 mg/ml
Gene Full Name dynactin 1|dynactin subunit 1
Alias Symbols 150 kDa dynein-associated polypeptide;AL022633;DAP-150;DP-150;dynactin 1 (p150, glued homolog, Drosophila);dynactin 1, retrograde axonal transport;dynactin subunit 1;Glue;Glued;P135;p15;p150;p150;p150-glued.
Peptide Sequence C-QEQLHQLHSRLIS
Product Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reference * Bupha-Intr T, Haizlip KM, Janssen PM.; Temporal changes in expression of connexin 43 after load-induced hypertrophy in vitro.; Am J Physiol Heart Circ Physiol. 2009 Mar;296(3):H806-14.; Pubmed 19136602
* Magnani E, Fan J, Gasparini L, Golding M, Williams M, Schiavo G, Goedert M, Amos LA, Spillantini MG.; Interaction of tau protein with the dynactin complex.; EMBO J. 2007 Oct 31;26(21):4546-54.; Pubmed 17932487
* Holzbaur EL, Tokito MK.; Localization of the DCTN1 gene encoding p150Glued to human chromosome 2p13 by fluorescence in situ hybridization.; Genomics. 1996 Feb 1;31(3):398-9. No abstract available.; Pubmed 8838327
Reconstitution and Storage Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals Printable datasheet for DCTN1 Antibody (OAEB02346)
Application Info Peptide ELISA: antibody detection limit dilution 1:128000.
Western Blot: Approx 150kDa band observed in cell lline A549 and in Human Testis lysates (calculated MW of 142kDa according to NP_004073.2). Recommended for use at 0.5-2ug/ml.
Immunohistochemistry: In paraffin embedded Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells.
In paraffin embedded Human Testis shows cytoplasm staining in cells of the seminiferous tubules. Recommended concentration, 2-4ug/ml.
Immunogen DCTN1
Uniprot ID Q14203
Protein Name Dynactin subunit 1
Protein Accession # NP_004073.2
Purification Ammonium Sulfate Precipitated; Affinity Purified
Gene Symbol Dctn1|DCTN1
Predicted Species Reactivity Human|Mouse
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Cow: 92%; Dog: 92%; Guinea Pig: 92%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 92%; Rat: 92%
Image 1
Human Cerebellum
OAEB02346 (2.5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Image 2
Human Testis
OAEB02346 staining (1ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
 

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