Product Number |
OAEB02346 |
Product Page |
www.avivasysbio.com/dctn1-antibody-oaeb02346.html |
Name |
DCTN1 Antibody (OAEB02346) |
Molecular Weight |
142; 127; 139; 127, 136, 140 |
NCBI Gene Id |
13191|1639 |
Host |
Goat |
Clonality |
Polyclonal |
Purity |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Concentration |
0.5 mg/ml |
Gene Full Name |
dynactin 1|dynactin subunit 1 |
Alias Symbols |
150 kDa dynein-associated polypeptide;AL022633;DAP-150;DP-150;dynactin 1 (p150, glued homolog, Drosophila);dynactin 1, retrograde axonal transport;dynactin subunit 1;Glue;Glued;P135;p15;p150;p150;p150-glued. |
Peptide Sequence |
C-QEQLHQLHSRLIS |
Product Format |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Reference |
* Bupha-Intr T, Haizlip KM, Janssen PM.; Temporal changes in expression of connexin 43 after load-induced hypertrophy in vitro.; Am J Physiol Heart Circ Physiol. 2009 Mar;296(3):H806-14.; Pubmed 19136602 * Magnani E, Fan J, Gasparini L, Golding M, Williams M, Schiavo G, Goedert M, Amos LA, Spillantini MG.; Interaction of tau protein with the dynactin complex.; EMBO J. 2007 Oct 31;26(21):4546-54.; Pubmed 17932487 * Holzbaur EL, Tokito MK.; Localization of the DCTN1 gene encoding p150Glued to human chromosome 2p13 by fluorescence in situ hybridization.; Genomics. 1996 Feb 1;31(3):398-9. No abstract available.; Pubmed 8838327 |
Reconstitution and Storage |
Aliquot and store at -20C. Minimize freezing and thawing. |
Datasheets/Manuals |
Printable datasheet for DCTN1 Antibody (OAEB02346) |
Application Info |
Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 150kDa band observed in cell lline A549 and in Human Testis lysates (calculated MW of 142kDa according to NP_004073.2). Recommended for use at 0.5-2ug/ml. Immunohistochemistry: In paraffin embedded Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells. In paraffin embedded Human Testis shows cytoplasm staining in cells of the seminiferous tubules. Recommended concentration, 2-4ug/ml. |
Immunogen |
DCTN1 |
Uniprot ID |
Q14203 |
Protein Name |
Dynactin subunit 1 |
Protein Accession # |
NP_004073.2 |
Purification |
Ammonium Sulfate Precipitated; Affinity Purified |
Gene Symbol |
Dctn1|DCTN1 |
Predicted Species Reactivity |
Human|Mouse |
Application |
WB, IHC |
Predicted Homology Based on Immunogen Sequence |
Cow: 92%; Dog: 92%; Guinea Pig: 92%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 92%; Rat: 92% |
Image 1 | Human Cerebellum
| OAEB02346 (2.5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
| Image 2 | Human Testis
| OAEB02346 staining (1ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
|
|