VPS28 Antibody (OAEB02343)

Data Sheet
 
Product Number OAEB02343
Product Page www.avivasysbio.com/vps28-antibody-oaeb02343.html
Name VPS28 Antibody (OAEB02343)
Molecular Weight 25.4
NCBI Gene Id 51160|66914
Host Goat
Clonality Polyclonal
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration 0.5 mg/ml
Gene Full Name vacuolar protein sorting 28|VPS28 subunit of ESCRT-I
Alias Symbols 1110014J03Rik;caspase-activated DNase inhibitor that interacts with ASK1;CIIA;D730005C08Rik;ESCRT-I complex subunit VPS28;vacuolar protein sorting 28 homolog;vacuolar protein sorting 28-like protein;vacuolar protein sorting-associated protein 28 homolog;VPS28, ESCRT-I subunit;yeast class E protein Vps28p homolog.
Peptide Sequence C-ESAYNAFNRFLHA
Product Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reference * Bishop N, Woodman P.; TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes.; J Biol Chem. 2001 Apr 13;276(15):11735-42.; Pubmed 11134028
Reconstitution and Storage Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals Printable datasheet for VPS28 Antibody (OAEB02343)
Application Info Peptide ELISA: antibody detection limit dilution 1:1000.
Western Blot: Approx 24kDa band observed in H460, Human Testis and Brain lysates (predicted MW of 25kDa according to NP_057292.1). Recommended for use at 1-3ug/ml.
Immunohistochemistry: In paraffin embedded Human Testis, stains acrosomal head caps in round spermatids. Recommended concentration, 10ug/ml.
Immunogen VPS28
Uniprot ID Q548N1
Protein Name Vacuolar protein sorting 28 (Yeast), isoform CRA_a EMBL EAW82094.1
Protein Accession # NP_057292.1
Purification Ammonium Sulfate Precipitated; Affinity Purified
Gene Symbol Vps28|VPS28
Predicted Species Reactivity Dog|Human|Mouse|Porcine|Rat
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
Image 1
Human Testis
OAEB02343 (10ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Image 2
Human Testis
OAEB02343 staining (1ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
 

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