Product Number |
OAEB02343 |
Product Page |
www.avivasysbio.com/vps28-antibody-oaeb02343.html |
Name |
VPS28 Antibody (OAEB02343) |
Molecular Weight |
25.4 |
NCBI Gene Id |
51160|66914 |
Host |
Goat |
Clonality |
Polyclonal |
Purity |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Concentration |
0.5 mg/ml |
Gene Full Name |
vacuolar protein sorting 28|VPS28 subunit of ESCRT-I |
Alias Symbols |
1110014J03Rik;caspase-activated DNase inhibitor that interacts with ASK1;CIIA;D730005C08Rik;ESCRT-I complex subunit VPS28;vacuolar protein sorting 28 homolog;vacuolar protein sorting 28-like protein;vacuolar protein sorting-associated protein 28 homolog;VPS28, ESCRT-I subunit;yeast class E protein Vps28p homolog. |
Peptide Sequence |
C-ESAYNAFNRFLHA |
Product Format |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Reference |
* Bishop N, Woodman P.; TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes.; J Biol Chem. 2001 Apr 13;276(15):11735-42.; Pubmed 11134028 |
Reconstitution and Storage |
Aliquot and store at -20C. Minimize freezing and thawing. |
Datasheets/Manuals |
Printable datasheet for VPS28 Antibody (OAEB02343) |
Application Info |
Peptide ELISA: antibody detection limit dilution 1:1000. Western Blot: Approx 24kDa band observed in H460, Human Testis and Brain lysates (predicted MW of 25kDa according to NP_057292.1). Recommended for use at 1-3ug/ml. Immunohistochemistry: In paraffin embedded Human Testis, stains acrosomal head caps in round spermatids. Recommended concentration, 10ug/ml. |
Immunogen |
VPS28 |
Uniprot ID |
Q548N1 |
Protein Name |
Vacuolar protein sorting 28 (Yeast), isoform CRA_a EMBL EAW82094.1 |
Protein Accession # |
NP_057292.1 |
Purification |
Ammonium Sulfate Precipitated; Affinity Purified |
Gene Symbol |
Vps28|VPS28 |
Predicted Species Reactivity |
Dog|Human|Mouse|Porcine|Rat |
Application |
WB, IHC |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100% |
Image 1 | Human Testis
| OAEB02343 (10ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. |
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Image 2 | Human Testis
| OAEB02343 staining (1ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
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