| Product Number |
OAEB02324 |
| Product Page |
www.avivasysbio.com/orc4-antibody-oaeb02324.html |
| Name |
ORC4 Antibody (OAEB02324) |
| Molecular Weight |
50.4; 40.9; 42.0 |
| NCBI Gene Id |
26428|295596|5000 |
| Host |
Goat |
| Clonality |
Polyclonal |
| Purity |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
origin recognition complex subunit 4|origin recognition complex, subunit 4 |
| Alias Symbols |
mMmOR;mMmORC4;Or;Orc4l;Orc4P;origin recognition complex subunit 4;origin recognition complex, subunit 4 homolog. |
| Peptide Sequence |
C-DVRQWATSSLSWL |
| Product Format |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reference |
* Johmura Y, Osada S, Nishizuka M, Imagawa M.; FAD24 acts in concert with histone acetyltransferase HBO1 to promote adipogenesis by controlling DNA replication.; J Biol Chem. 2008 Jan 25;283(4):2265-74.; Pubmed 18029353
* Johmura Y, Osada S, Nishizuka M, Imagawa M.; FAD24 acts in concert with histone acetyltransferase HBO1 to promote adipogenesis by controlling DNA replication.; J Biol Chem 283:2265-74 (2008).; Pubmed 18029353
* Radichev I, Kwon SW, Zhao Y, DePamphilis ML, Vassilev A.; Genetic analysis of human Orc2 reveals specific domains that are required in vivo for assembly and nuclear localization of the origin recognition complex.; J Biol Chem. 2006 Aug 11;281(32):23264-73.; Pubmed 16762929
* Radichev I, Kwon SW, Zhao Y, DePamphilis ML, Vassilev A.; Genetic analysis of human Orc2 reveals specific domains that are required in vivo for assembly and nuclear localization of the origin recognition complex.; J Biol Chem 281:23264-73 (2006).; Pubmed 16762929
* Todorovic V, Giadrossi S, Pelizon C, Mendoza-Maldonado R, Masai H, Giacca M.; Human origins of DNA replication selected from a library of nascent DNA.; Mol Cell. 2005 Aug 19;19(4):567-75.; Pubmed 16109380
* Vashee S, Simancek P, Challberg MD, Kelly TJ.; Assembly of the human origin recognition complex.; J Biol Chem 2001 Jul 13;276(28):26666-73; Pubmed 11323433 |
| Reconstitution and Storage |
Aliquot and store at -20C. Minimize freezing and thawing. |
| Datasheets/Manuals |
Printable datasheet for ORC4 Antibody (OAEB02324) |
| Additional Information |
Reactivity Info: Tested: Human, Mouse; Expected: Human, Mouse, Rat, Dog, Cow |
| Application Info |
Peptide ELISA: antibody detection limit dilution 1:8000.
Western Blot: Approx 50kDa band observed in Human and Mouse Heart lysates (calculated MW of 50.4kDa according to Human NP_002543.2 and of 50.0kDa according to Mouse NP_036088.3). Recommended concentration: 0.5-2ug/ml.
Other Notes: ChIP: reported by a customer to work very well in ChIP. |
| Immunogen |
ORC4 |
| Uniprot ID |
O43929 |
| Protein Name |
Origin recognition complex subunit 4 |
| Protein Accession # |
NP_002543.2 |
| Purification |
Ammonium Sulfate Precipitated; Affinity Purified |
| Gene Symbol |
Orc4|ORC4 |
| Predicted Species Reactivity |
Bovine|Dog|Human|Mouse|Rat |
| Application |
WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100% |
| Image 1 | Rat Heart
 | OAEB02324 (0.5ug/ml) staining of Rat Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
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