Goat Anti-ERK2 / MAPK1 Antibody (OAEB02222)

Data Sheet
 
Product Number OAEB02222
Product Page www.avivasysbio.com/goat-anti-erk2-mapk1-antibody-oaeb02222.html
Name Goat Anti-ERK2 / MAPK1 Antibody (OAEB02222)
Molecular Weight 41.4; 41.4
NCBI Gene Id 26413|5594
Host Goat
Clonality Polyclonal
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration 0.5 mg/ml
Gene Full Name mitogen-activated protein kinase 1
Alias Symbols 9030612K14Rik;AA407128;AU018647;C78273;ERK;Erk2;ERK-2;ERT1;extracellular signal-regulated kinase 2;MAP;MAP kinase 1;MAP kinase 2;MAPK 1;MAPK 2;MAPK2;mitogen-activated protein kinase 1;mitogen-activated protein kinase 2;NS13;p38;p40;p41;p41mapk;p42m;p42mapk;p42-MAPK;Prk;Prkm1;PRKM2;protein tyrosine kinase ERK2.
Peptide Sequence CAAGPEMVRGQVF
Product Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reference * Lee SM, Nguyen TH, Park MH, Kim KS, Cho KJ, Moon DC, Kim HY, Yoon do Y, Hong JT.; EPO receptor-mediated ERK kinase and NF-kappaB activation in erythropoietin-promoted differentiation of astrocytes.; Biochem Biophys Res Commun. 2004 Aug 6;320(4):1087-95.; Pubmed 15249201
Reconstitution and Storage Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals Printable datasheet for Goat Anti-ERK2 / MAPK1 Antibody (OAEB02222)
Application Info Peptide ELISA: antibody detection limit dilution 1:8000.
Western Blot: Approx 38kDa band observed in human cervix epitheloid carcinoma HeLa and and human hepatoblastoma HepG2 lysates (calculated MW of 41.4kDa according to NP_002736 and NP_620407). Recommended concentration: 0.5-2ug/ml.
Immunogen MAPK1
Uniprot ID P28482
Protein Name Mitogen-activated protein kinase 1
Protein Accession # NP_002736.3
Purification Ammonium Sulfate Precipitated; Affinity Purified
Gene Symbol Mapk1|MAPK1
Predicted Species Reactivity Bovine|Dog|Human|Mouse|Rat
Application WB
Predicted Homology Based on Immunogen Sequence Dog: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100%
Image 1
HepG2
OAEB02222 (0.5ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 

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