Product Number |
OAEB02031 |
Product Page |
www.avivasysbio.com/igfbp3-antibody-oaeb02031.html |
Name |
IGFBP3 Antibody - middle region (OAEB02031) |
Molecular Weight |
31.7; 32.2 |
NCBI Gene Id |
16009|24484|3486 |
Host |
Goat |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
insulin like growth factor binding protein 3|insulin-like growth factor binding protein 3 |
Alias Symbols |
acid stable subunit of the 140 K IGF complex;AI649005;binding protein 29;binding protein 53;BP-53;growth hormone-dependent binding protein;IBP3;IBP-3;IGF-binding protein 3;IGFBP;IGFBP-3;IGF-BP3;IGgfbp3;insulin-like growth factor-binding protein (IGF-BP3);insulin-like growth factor-binding protein 3. |
Peptide Sequence |
C-RYKVDYESQSTDTQN |
Product Format |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Reference |
* Lin J, Manson JE, Lee IM, Cook NR, Buring JE, Zhang SM.; Intakes of calcium and vitamin D and breast cancer risk in women.; Arch Intern Med. 2007 May 28;167(10):1050-9.; Pubmed 17533208 |
Reconstitution and Storage |
Aliquot and store at -20C. Minimize freezing and thawing. |
Datasheets/Manuals |
Printable datasheet for IGFBP3 Antibody - middle region (OAEB02031) |
Specificity |
This antibody is expected to recognise both reported isoforms (NP_001013416.1 and NP_000589.2). |
Application Info |
Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Approx 28kDa band observed in Human Breast cancer lysates (calculated MW of 31.7kDa according to NP_001013416.1 and 32.2kDa according to NP_000589.2). Recommended concentration: 0.02-0.06ug/ml. An additional band of 50kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot. |
Immunogen |
IGFBP3 |
Uniprot ID |
P17936 |
Protein Name |
Insulin-like growth factor-binding protein 3 |
Protein Accession # |
NP_001013416.1 |
Purification |
Ammonium Sulfate Precipitated; Affinity Purified |
Gene Symbol |
Igfbp3|IGFBP3 |
Predicted Species Reactivity |
Dog|Human|Mouse|Rat |
Application |
WB |
Predicted Homology Based on Immunogen Sequence |
Dog: 100%; Goat: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Sheep: 100% |
Image 1 | Human Breast cancer
| OAEB02031 (0.02ug/ml) staining of Human Breast cancer lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence. |
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