IGFBP3 Antibody - middle region (OAEB02031)

Data Sheet
 
Product Number OAEB02031
Product Page www.avivasysbio.com/igfbp3-antibody-oaeb02031.html
Name IGFBP3 Antibody - middle region (OAEB02031)
Molecular Weight 31.7; 32.2
NCBI Gene Id 16009|24484|3486
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name insulin like growth factor binding protein 3|insulin-like growth factor binding protein 3
Alias Symbols acid stable subunit of the 140 K IGF complex;AI649005;binding protein 29;binding protein 53;BP-53;growth hormone-dependent binding protein;IBP3;IBP-3;IGF-binding protein 3;IGFBP;IGFBP-3;IGF-BP3;IGgfbp3;insulin-like growth factor-binding protein (IGF-BP3);insulin-like growth factor-binding protein 3.
Peptide Sequence C-RYKVDYESQSTDTQN
Product Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reference * Lin J, Manson JE, Lee IM, Cook NR, Buring JE, Zhang SM.; Intakes of calcium and vitamin D and breast cancer risk in women.; Arch Intern Med. 2007 May 28;167(10):1050-9.; Pubmed 17533208
Reconstitution and Storage Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals Printable datasheet for IGFBP3 Antibody - middle region (OAEB02031)
Specificity This antibody is expected to recognise both reported isoforms (NP_001013416.1 and NP_000589.2).
Application Info Peptide ELISA: antibody detection limit dilution 1:64000.
Western Blot: Approx 28kDa band observed in Human Breast cancer lysates (calculated MW of 31.7kDa according to NP_001013416.1 and 32.2kDa according to NP_000589.2). Recommended concentration: 0.02-0.06ug/ml.
An additional band of 50kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Immunogen IGFBP3
Uniprot ID P17936
Protein Name Insulin-like growth factor-binding protein 3
Protein Accession # NP_001013416.1
Purification Ammonium Sulfate Precipitated; Affinity Purified
Gene Symbol Igfbp3|IGFBP3
Predicted Species Reactivity Dog|Human|Mouse|Rat
Application WB
Predicted Homology Based on Immunogen Sequence Dog: 100%; Goat: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Sheep: 100%
Image 1
Human Breast cancer
OAEB02031 (0.02ug/ml) staining of Human Breast cancer lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
 

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