| Product Number |
OAEB01786 |
| Product Page |
www.avivasysbio.com/goat-anti-siahbp1-fir-antibody-oaeb01786.html |
| Name |
Goat Anti-SIAHBP1 / FIR Antibody (OAEB01786) |
| Molecular Weight |
59.9; 58.2; 55.7 |
| NCBI Gene Id |
22827|67959 |
| Host |
Goat |
| Clonality |
Polyclonal |
| Purity |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
poly(U) binding splicing factor 60|poly-U binding splicing factor 60 |
| Alias Symbols |
2410104I19Rik;2810454F19Rik;60 kDa poly(U)-binding-splicing factor;FBP interacting repressor;FIR;fuse-binding protein-interacting repressor;poly(U) binding splicing factor 60KDa;poly(U)-binding-splicing factor PUF60;pyrimidine tract binding splicing factor;Ro ribonucleoprotein-binding protein 1;RoBPI;Siah binding protein 1;SIAHBP1;VRJS. |
| Peptide Sequence |
C-YDQERFDNSDLSA |
| Product Format |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reference |
* Corsini L, Hothorn M, Stier G, Rybin V, Scheffzek K, Gibson TJ, Sattler M.; Dimerization and protein binding specificity of the U2AF homology motif of the splicing factor Puf60.; J Biol Chem. 2009 Jan 2;284(1):630-9.; Pubmed 18974054
* Liu J, He L, Collins I, Ge H, Libutti D, Li J, Egly JM, Levens D.; The FBP interacting repressor targets TFIIH to inhibit activated transcription.; Mol Cell. 2000 Feb;5(2):331-41.; Pubmed 10882074 |
| Reconstitution and Storage |
Aliquot and store at -20C. Minimize freezing and thawing. |
| Datasheets/Manuals |
Printable datasheet for Goat Anti-SIAHBP1 / FIR Antibody (OAEB01786) |
| Application Info |
Peptide ELISA: antibody detection limit dilution 1:32000.
Western Blot: Approx 60kDa band observed in lysate of human epidermoid carcinoma A431 (calculated MW of 59.9kDa (isoform A) according to NP_510965.1 and 58.2kDa (isoform B) according to NP_055096.2). Recommended concentration: 1-3ug/ml. |
| Immunogen |
PUF60 |
| Uniprot ID |
Q9UHX1 |
| Protein Name |
Poly(U)-binding-splicing factor PUF60 |
| Protein Accession # |
NP_510965.1 |
| Purification |
Ammonium Sulfate Precipitated; Affinity Purified |
| Gene Symbol |
Puf60|PUF60 |
| Predicted Species Reactivity |
Bovine|Dog|Human|Mouse|Rat |
| Application |
WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 100% |
| Image 1 | A431
 | OAEB01786 (1ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
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