Goat Anti-FAS / CD95 Antibody (OAEB01680)

Data Sheet
 
Product Number OAEB01680
Product Page www.avivasysbio.com/goat-anti-fas-cd95-antibody-oaeb01680.html
Name Goat Anti-FAS / CD95 Antibody (OAEB01680)
Molecular Weight 37.7; 35.4; 24.8; 16.6; 14.6; 9.39; 11.4
NCBI Gene Id 355
Host Goat
Clonality Polyclonal
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration 0.5 mg/ml
Gene Full Name Fas cell surface death receptor
Alias Symbols ALPS1A;APO-1;APO-1 cell surface antigen;apoptosis antigen 1;apoptosis signaling receptor FAS;apoptosis-mediating surface antigen FAS;APT1;CD95;CD95 antigen;Fas (TNF receptor superfamily, member 6);Fas AMA;FAS1;FASLG receptor;FASTM;mutant tumor necrosis receptor superfamily member 6;TNF receptor superfamily member 6;TNFRSF6;tumor necrosis factor receptor superfamily member 6;tumor necrosis factor receptor superfamily, member 6.
Peptide Sequence KTCRKHRKENQGSH
Product Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reference * Blomberg J, Ruuth K, Jacobsson M, Hoglund A, Nilsson JA, Lundgren E,; Reduced FAS transcription in clones of U937 cells that have acquired resistance to Fas-induced apoptosis.; FEBS J. 2009 Jan 276 (2): 497-508; Pubmed 19076218
Reconstitution and Storage Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals Printable datasheet for Goat Anti-FAS / CD95 Antibody (OAEB01680)
Additional Information Reactivity Info: Tested: Human; Expected: Human
Application Info Peptide ELISA: antibody detection limit dilution 1:32000.
Western Blot: Approx 37kDa band observed in lysates of cell line MOLT4 (calculated MW of 37.7kDa according to NP_000034.1). Recommended concentration: 0.1-0.3ug/ml.
IHC:Paraffin embedded Human Tonsil and Small Intestine. Recommended concentration: 3.75ug/ml.
Immunogen FAS
Uniprot ID P25445
Protein Name Tumor necrosis factor receptor superfamily member 6
Protein Accession # NP_000034.1
Purification Ammonium Sulfate Precipitated; Affinity Purified
Gene Symbol FAS
Predicted Species Reactivity Human
Application WB
Predicted Homology Based on Immunogen Sequence Human: 100%
Image 1
MOLT4
OAEB01680 (0.1ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Image 2
Human Tonsil
OAEB01680 staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
 

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