Product Number |
OAEB01633 |
Product Page |
www.avivasysbio.com/siah1-antibody-oaeb01633.html |
Name |
SIAH1 Antibody (OAEB01633) |
Molecular Weight |
31.1; 34.6 |
NCBI Gene Id |
140941|20437|6477 |
Host |
Goat |
Clonality |
Polyclonal |
Purity |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Concentration |
0.5 mg/ml |
Gene Full Name |
siah E3 ubiquitin protein ligase 1|siah E3 ubiquitin protein ligase 1A |
Alias Symbols |
AA982064;AI853500;BURHAS;E3 ubiquitin-protein ligase SIAH1;E3 ubiquitin-protein ligase SIAH1A;mSiah-1a;RING-type E3 ubiquitin transferase SIAH1;RING-type E3 ubiquitin transferase SIAH1A;seven in absentia 1A;seven in absentia homolog 1;seven in absentia homolog 1a;Si;SIAH;siah-1;siah-1a;SIAH1A;Sinh1a. |
Peptide Sequence |
SRQTATALPTGTSKC |
Product Format |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Reference |
* Guigon CJ, Zhao L, Lu C, Willingham MC, Cheng SY.; Regulation of beta-catenin by a novel nongenomic action of thyroid hormone beta receptor.; Mol Cell Biol. 2008 Jul;28(14):4598-608.; Pubmed 18474620 * Groenman F, Rutter M, Caniggia I, Tibboel D, Post M.; Hypoxia-inducible factors in the first trimester human lung.; J Histochem Cytochem. 2007 Apr;55(4):355-63.; Pubmed 17189520 * Iwai A, Marusawa H, Matsuzawa S, Fukushima T, Hijikata M, Reed JC, Shimotohno; K, Chiba T.; Siah-1L, a novel transcript variant belonging to the human Siah family of proteins, regulates beta-catenin activity in a p53-dependent manner.; Oncogene. 2004 Sep 30;23(45):7593-600.; Pubmed 15326481 * Susini L, Passer BJ, Amzallag-Elbaz N, Juven-Gershon T, Prieur S, Privat N, Tuynder M, Gendron MC, Israel A, Amson R, Oren M, Telerman A.; Siah-1 binds and regulates the function of Numb.; Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15067-72.; Pubmed 11752454 |
Reconstitution and Storage |
Aliquot and store at -20C. Minimize freezing and thawing. |
Datasheets/Manuals |
Printable datasheet for SIAH1 Antibody (OAEB01633) |
Application Info |
Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 28+30kDa bands observed in Human Liver lysates (calculated MW of 31.1kDa and 34.6kDa according to NP_003022.3 and NP_001006611.1). Recommended concentration: 0.1-0.3ug/ml. |
Immunogen |
SIAH1 |
Additional Information |
Description: This product is expected to recognize both reported isoforms (according to NP_003022.3; NP_001006611.1) |
Uniprot ID |
Q8IUQ4 |
Protein Name |
E3 ubiquitin-protein ligase SIAH1 |
Protein Accession # |
NP_003022.3 |
Purification |
Ammonium Sulfate Precipitated; Affinity Purified |
Gene Symbol |
Siah1|SIAH1|Siah1a |
Predicted Species Reactivity |
Bovine|Dog|Human|Mouse|Rat |
Application |
WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 86%; Rat: 100%; Zebrafish: 100% |
Image 1 | Human Liver
| OAEB01633 staining (1ug/ml) of Human Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.chemiluminescence. |
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Image 2 | Immunofluorescence
| Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing mitochondrial/cytoplasmic and nuclear staining. Th |
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