SIAH1 Antibody (OAEB01633)

Data Sheet
 
Product Number OAEB01633
Product Page www.avivasysbio.com/siah1-antibody-oaeb01633.html
Name SIAH1 Antibody (OAEB01633)
Molecular Weight 31.1; 34.6
NCBI Gene Id 140941|20437|6477
Host Goat
Clonality Polyclonal
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration 0.5 mg/ml
Gene Full Name siah E3 ubiquitin protein ligase 1|siah E3 ubiquitin protein ligase 1A
Alias Symbols AA982064;AI853500;BURHAS;E3 ubiquitin-protein ligase SIAH1;E3 ubiquitin-protein ligase SIAH1A;mSiah-1a;RING-type E3 ubiquitin transferase SIAH1;RING-type E3 ubiquitin transferase SIAH1A;seven in absentia 1A;seven in absentia homolog 1;seven in absentia homolog 1a;Si;SIAH;siah-1;siah-1a;SIAH1A;Sinh1a.
Peptide Sequence SRQTATALPTGTSKC
Product Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reference * Guigon CJ, Zhao L, Lu C, Willingham MC, Cheng SY.; Regulation of beta-catenin by a novel nongenomic action of thyroid hormone beta receptor.; Mol Cell Biol. 2008 Jul;28(14):4598-608.; Pubmed 18474620
* Groenman F, Rutter M, Caniggia I, Tibboel D, Post M.; Hypoxia-inducible factors in the first trimester human lung.; J Histochem Cytochem. 2007 Apr;55(4):355-63.; Pubmed 17189520
* Iwai A, Marusawa H, Matsuzawa S, Fukushima T, Hijikata M, Reed JC, Shimotohno; K, Chiba T.; Siah-1L, a novel transcript variant belonging to the human Siah family of proteins, regulates beta-catenin activity in a p53-dependent manner.; Oncogene. 2004 Sep 30;23(45):7593-600.; Pubmed 15326481
* Susini L, Passer BJ, Amzallag-Elbaz N, Juven-Gershon T, Prieur S, Privat N, Tuynder M, Gendron MC, Israel A, Amson R, Oren M, Telerman A.; Siah-1 binds and regulates the function of Numb.; Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15067-72.; Pubmed 11752454
Reconstitution and Storage Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals Printable datasheet for SIAH1 Antibody (OAEB01633)
Application Info Peptide ELISA: antibody detection limit dilution 1:32000.
Western Blot: Approx 28+30kDa bands observed in Human Liver lysates (calculated MW of 31.1kDa and 34.6kDa according to NP_003022.3 and NP_001006611.1). Recommended concentration: 0.1-0.3ug/ml.
Immunogen SIAH1
Additional Information Description: This product is expected to recognize both reported isoforms (according to NP_003022.3; NP_001006611.1)
Uniprot ID Q8IUQ4
Protein Name E3 ubiquitin-protein ligase SIAH1
Protein Accession # NP_003022.3
Purification Ammonium Sulfate Precipitated; Affinity Purified
Gene Symbol Siah1|SIAH1|Siah1a
Predicted Species Reactivity Bovine|Dog|Human|Mouse|Rat
Application WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 86%; Rat: 100%; Zebrafish: 100%
Image 1
Human Liver
OAEB01633 staining (1ug/ml) of Human Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.chemiluminescence.
Image 2
Immunofluorescence
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing mitochondrial/cytoplasmic and nuclear staining. Th
 

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