Product Number |
OAEB01630 |
Product Page |
www.avivasysbio.com/goat-anti-kip1-cdkn1b-internal-antibody-oaeb01630.html |
Name |
Goat Anti-KIP1 / CDKN1B (internal) Antibody (OAEB01630) |
Molecular Weight |
22.1 |
NCBI Gene Id |
1027 |
Host |
Goat |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
cyclin dependent kinase inhibitor 1B |
Description |
|
Alias Symbols |
CDKN4;cyclin-dependent kinase inhibitor 1B;cyclin-dependent kinase inhibitor 1B (p27, Kip1);KIP1;MEN1B;MEN4;P27KIP1. |
Peptide Sequence |
C-KRPATDDSSTQNK |
Product Format |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Reconstitution and Storage |
Aliquot and store at -20C. Minimize freezing and thawing. |
Datasheets/Manuals |
Printable datasheet for Goat Anti-KIP1 / CDKN1B (internal) Antibody (OAEB01630) |
Application Info |
Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 26kDa band observed in lysates of Human Prostate and of Hepatoblastoma cell line HepG2 (calculated MW of 22.1kDa according to NP_004055.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xia et al, Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):14028-33; PMID: 16169901). Recommended concentration: 0.1-0.3 ug/ml. IHC: In paraffin embedded Human Prostate shows nuclear staining of secretory cells. Recommended concentration, 5-10 ug/ml. |
Immunogen |
CDKN1B |
Uniprot ID |
P46527 |
Protein Name |
Cyclin-dependent kinase inhibitor 1B |
Publications |
Differential regulation of Janus kinase 3 (JAK3) in bovine preovulatory follicles and identification of JAK3 interacting proteins in granulosa cells. J Ovarian Res. 9, 71 (2016). 27793176 |
Protein Accession # |
NP_004055.1 |
Purification |
Ammonium Sulfate Precipitated; Affinity Purified |
Gene Symbol |
CDKN1B |
Predicted Species Reactivity |
Bovine|Cat|Dog|Human|Mouse|Porcine|Rat |
Application |
P-WB, IHC |
Predicted Homology Based on Immunogen Sequence |
Human, Mouse, Rat, Cat, Dog, Pig, Cow |
Image 1 | Human Prostate
| OAEB01630 (5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
| Image 2 | Human Prostate
| OAEB01630 (0.1ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
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