| Product Number |
OAEB01425 |
| Product Page |
www.avivasysbio.com/trim2-antibody-oaeb01425.html |
| Name |
TRIM2 Antibody (OAEB01425) |
| Molecular Weight |
84.5; 81.5; 83.9; 83.6; 81.8; 67.7 |
| NCBI Gene Id |
23321|80890 |
| Host |
Goat |
| Clonality |
Polyclonal |
| Purity |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
tripartite motif containing 2|tripartite motif-containing 2 |
| Alias Symbols |
CMT2R;E3 ubiquitin-protein ligase TRIM2;mKIAA0517;n;narf;neural activity-related ring finger protein;RING finger protein 86;RING-type E3 ubiquitin transferase TRIM2;RNF86;tripartite motif protein 2;tripartite motif protein TRIM2;tripartite motif-containing protein 2. |
| Peptide Sequence |
DSGNHCFKVYRYLQ |
| Product Format |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reference |
* Reymond A, Meroni G, Fantozzi A, Merla G, Cairo S, Luzi L, Riganelli D, Zanaria E, Messali S, Cainarca S, Guffanti A, Minucci S, Pelicci PG, Ballabio A.; The tripartite motif family identifies cell compartments.; EMBO J. 2001 May 1;20(9):2140-51.; Pubmed 11331580 |
| Reconstitution and Storage |
Aliquot and store at -20C. Minimize freezing and thawing. |
| Datasheets/Manuals |
Printable datasheet for TRIM2 Antibody (OAEB01425) |
| Application Info |
Peptide ELISA: antibody detection limit dilution 1:16000.
Western Blot: Approx 80+85kDa bands observed in Rat Brain lysates (calculated MW of 81.5kDa according to Human NP_001123539.1, 84.4 accorindg to Human NP_056086.2 and 83.3 according to Rat NP_001102022.1). Recommended concentration: 0.5-2ug/ml..
An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptide. |
| Immunogen |
TRIM2 |
| Uniprot ID |
Q9C040 |
| Protein Name |
Tripartite motif-containing protein 2 |
| Protein Accession # |
NP_056086.2 |
| Purification |
Ammonium Sulfate Precipitated; Affinity Purified |
| Gene Symbol |
Trim2|TRIM2 |
| Predicted Species Reactivity |
Bovine|Dog|Human|Mouse|Rat |
| Application |
WB, IHC |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100% |
| Image 1 | Human Cortex
 | OAEB01425 (6.3ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
|
| Image 2 | Rat Brain
 | OAEB01425 (0.5ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
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