| Product Number |
OAEB01299 |
| Product Page |
www.avivasysbio.com/ran-antibody-oaeb01299.html |
| Name |
RAN Antibody (OAEB01299) |
| Molecular Weight |
24.4 |
| NCBI Gene Id |
19384|5901|84509 |
| Host |
Goat |
| Clonality |
Polyclonal |
| Purity |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
RAN, member RAS oncogene family |
| Alias Symbols |
androgen receptor-associated protein 24;ARA24;Gsp1;GTPase Ran;GTP-binding nuclear protein Ran;guanosine triphosphatase Ran;member RAS oncogene family;RanGTPase;ras-like protein TC4;ras-related nuclear protein;TC4. |
| Peptide Sequence |
AAQGEPQVQFKLV-C |
| Product Format |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reference |
* Ren M, Drivas G, D'Eustachio P, Rush MG.; Ran/TC4: a small nuclear GTP-binding protein that regulates DNA synthesis.; J Cell Biol. 1993 Jan;120(2):313-23.; Pubmed 8421051 |
| Reconstitution and Storage |
Aliquot and store at -20C. Minimize freezing and thawing. |
| Datasheets/Manuals |
Printable datasheet for RAN Antibody (OAEB01299) |
| Application Info |
Peptide ELISA: antibody detection limit dilution 1:16000.
Western Blot: Approx 25kDa band observed in Jurkat and A431 lysates (predicted MW of 25kDa according to NP_006316). Recommended for use at 1-3ug/ml. |
| Immunogen |
RAN |
| Uniprot ID |
P62826 |
| Protein Name |
GTP-binding nuclear protein Ran |
| Protein Accession # |
NP_006316.1 |
| Purification |
Ammonium Sulfate Precipitated; Affinity Purified |
| Gene Symbol |
Ran|RAN |
| Predicted Species Reactivity |
Bovine|Dog|Human|Mouse|Rat |
| Application |
WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 83% |
| Image 1 | A431
 | OAEB01299 (0.5ug/ml) staining of A431 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
|
| Image 2 | Immunohistochemistry
 | (3ug/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. |
|
| Image 3 | Immunohistochemistry
 | Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody. |
|
| Image 4 | Western Blot
 | (0.5ug/ml) staining of A431 (35ug protein in RIPA buffer). Detected by chemiluminescence. |
|
