| Product Number |
OAEB01260 |
| Product Page |
www.avivasysbio.com/goat-anti-lig1-dna-ligase-i-antibody-oaeb01260.html |
| Name |
Goat Anti-LIG1 / DNA ligase I Antibody (OAEB01260) |
| Molecular Weight |
102 |
| NCBI Gene Id |
3978|81513 |
| Host |
Goat |
| Clonality |
Polyclonal |
| Purity |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
DNA ligase 1 |
| Alias Symbols |
DNA ligase (ATP) 1;DNA ligase 1;DNA ligase I;ligase I, DNA, ATP-dependent;polydeoxyribonucleotide synthase [ATP] 1. |
| Peptide Sequence |
C-RVREDKQPEQATTS |
| Product Format |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reference |
* Ferrari G, Rossi R, Arosio D, Vindigni A, Biamonti G, Montecucco A,; Cell cycle-dependent phosphorylation of human DNA ligase I at the cyclin-dependent kinase sites.; J. Biol. Chem. 2003 Sep 278 (39): 37761-7.; Pubmed 12851383 |
| Reconstitution and Storage |
Aliquot and store at -20C. Minimize freezing and thawing. |
| Datasheets/Manuals |
Printable datasheet for Goat Anti-LIG1 / DNA ligase I Antibody (OAEB01260) |
| Application Info |
Peptide ELISA: antibody detection limit dilution 1:16000.
Western Blot: Approx 125kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 102kDa according to NP_000225.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml. |
| Immunogen |
LIG1 |
| Uniprot ID |
P18858 |
| Protein Name |
DNA ligase 1 |
| Protein Accession # |
NP_000225.1 |
| Purification |
Ammonium Sulfate Precipitated; Affinity Purified |
| Gene Symbol |
Lig1|LIG1 |
| Predicted Species Reactivity |
Human|Rat |
| Application |
WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 92%; Dog: 85%; Human: 100%; Mouse: 92%; Pig: 78%; Rat: 100% |
| Image 1 | HeLa nuclear, cytosolic
 | OAEB01260 (0.1ug/ml) staining of HeLa nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
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