| Product Number |
OAEB01045 |
| Product Page |
www.avivasysbio.com/goat-anti-sirtuin-1-sirt1-aa577-590-antibody-oaeb01045.html |
| Name |
Goat Anti-Sirtuin 1 / SIRT1 (aa577-590) Antibody (OAEB01045) |
| Molecular Weight |
81.7; 50.5 |
| NCBI Gene Id |
23411|93759 |
| Host |
Goat |
| Clonality |
Polyclonal |
| Purity |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
sirtuin 1 |
| Alias Symbols |
AA673258;NAD-dependent protein deacetylase sirtuin-1;NAD-dependent protein deacylase sirtuin-1;regulatory protein SIR2 homolog 1;S;Si;Sir2;Sir2a;Sir2alpha;SIR2L1;SIR2-like protein 1;sirtuin 1 ((silent mating type information regulation 2, homolog) 1;sirtuin type 1. |
| Peptide Sequence |
C-EKPQEVQTSRNVES |
| Product Format |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution and Storage |
Aliquot and store at -20C. Minimize freezing and thawing. |
| Datasheets/Manuals |
Printable datasheet for Goat Anti-Sirtuin 1 / SIRT1 (aa577-590) Antibody (OAEB01045) |
| Additional Information |
Epitope Info: internal region
Immunogen: Peptide with sequence C-EKPQEVQTSRNVES, from the internal region of the protein sequence according to NP_036370.2; NP_001135970.1. |
| Additional Information |
Note: This antibody is expected to recognize both reported isoforms (NP_036370.2; NP_001135970.1).
Reactivity Info: Tested: Human; Expected: Human, Mouse |
| Application Info |
Peptide ELISA: Antibody detection limit dilution 1:64000.
Western Blot: Approx 120kDa band observed in lysates of cell line Jurkat (calculated MW of 81.7kDa according to NP_036370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cheng et al, Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10794-9.; PMID: 12960381). Recommended concentration: 0.05-0.2ug/ml. |
| Immunogen |
SIRT1 |
| Uniprot ID |
Q96EB6 |
| Protein Name |
NAD-dependent protein deacetylase sirtuin-1 |
| Protein Accession # |
NP_036370.2 |
| Purification |
Ammonium Sulfate Precipitated; Affinity Purified |
| Gene Symbol |
Sirt1|SIRT1 |
| Predicted Species Reactivity |
Human|Mouse |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Guinea Pig: 86%; Human: 100%; Mouse: 100%; Pig: 78%; Rat: 85% |
| Image 1 | Human Skin
 | OAEB01045 (3.8ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
| | Image 2 | Jurkat
 | OAEB01045 (0.05ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
|
|
