CYP26B1 Antibody (OAEB00834)

Data Sheet
 
Product Number OAEB00834
Product Page www.avivasysbio.com/cyp26b1-antibody-oaeb00834.html
Name CYP26B1 Antibody (OAEB00834)
Molecular Weight 57.5; 49.0
NCBI Gene Id 232174|312495|56603
Host Goat
Clonality Polyclonal
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration 0.5 mg/ml
Gene Full Name cytochrome P450 family 26 subfamily B member 1|cytochrome P450, family 26, subfamily b, polypeptide 1
Alias Symbols CP2;CP26;CYP26A2;cytochrome P450 26B1;cytochrome P450 family 26 subfamily A member 1;cytochrome P450 retinoic acid-inactivating 2;cytochrome P450 retinoid metabolizing protein;cytochrome P450, 26, retinoic acid B1;cytochrome P450, family 26, subfamily B, polypeptide 1;cytochrome P450, subfamily XXVIB, polypeptide 1;cytochrome P450RAI-2;P450R;P450RAI2;P450RAI-2;retinoic acid B1;retinoic acid hydroxylase;retinoic acid-metabolizing cytochrome;RHFCA.
Peptide Sequence C-SQARSEDKDGRFH
Product Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reference Bowles J, Knight D, Smith C, Wilhelm D, Richman J, Mamiya S, Yashiro K, Chawengsaksophak K, Wilson MJ, Rossant J, Hamada H, Koopman P.
Retinoid signaling determines germ cell fate in mice.
Science. 2006 Apr 28;312(5773):596-600. Epub 2006 Mar 30. PMID: 16574820
Reconstitution and Storage Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals Printable datasheet for CYP26B1 Antibody (OAEB00834)
Additional Information Amino Acids: 512 aa
Additional Information Reactivity Info: Tested: Human; Expected: Human, Mouse, Rat, Dog
Application Info Peptide ELISA: Antibody detection limit dilution 1:128000.
Immunohistochemistry: In paraffin embedded Human Cerebellum shows staining of cell bodies in the Molecular Layer. Recommended concentration, 4-6ug/ml.
Western Blot: Preliminary experiments gave an approx 85kDa band in human testis lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 57.5kDa according to NP_063938.1. The 85kDa band was successfully blocked by incubation with the immunizing peptide.
Immunogen CYP26B1
Uniprot ID Q9NR63
Protein Name Cytochrome P450 26B1
Protein Accession # NP_063938.1
Purification Ammonium Sulfate Precipitated; Affinity Purified
Gene Symbol Cyp26b1|CYP26B1
Predicted Species Reactivity Dog|Human|Mouse|Rat
Application IHC
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Human: 100%; Mouse: 100%; Rat: 100%
Image 1
Human Cerebellum
OAEB00834 (4ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
 

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