Goat Anti-CIAS1 / cryopyrin (C Terminus) Antibody (OAEB00821)

• Product Number: OAEB00821
• Name: Goat Anti-CIAS1 / cryopyrin (C Terminus) Antibody (OAEB00821)
• Molecular Weight: 118; 106; 112
• NCBI Gene Id: 114548
• Host: Goat
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: NLR family pyrin domain containing 3
• Alias Symbols: AGTAVPRL;AII;AVP;C1orf7;caterpiller protein 1.1;CIAS1;CLR1.1;cold autoinflammatory syndrome 1 protein;cold-induced autoinflammatory syndrome 1 protein;cryopyrin;cryopyrin, NACHT, LRR and PYD domains - containing protein 3;deafness, autosomal dominant 34;DFNA34;FCAS;FCAS1;FCU;KEFH;MWS;NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3;NACHT, LRR and PYD containing protein 3;NACHT, LRR and PYD domains-containing protein 3;NALP3;nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3;PYPAF1;PYRIN-containing APAF1-like protein 1.
• Peptide Sequence: C-EKPELTVVFEPSW
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Reference: * Hoffman HM, Mueller JL, Broide DH, Wanderer AA, Kolodner RD.; Mutation of a new gene encoding a putative pyrin-like protein causes familial cold autoinflammatory syndrome and Muckle-Wells syndrome.; Nat Genet. 2001 Nov;29(3):301-5.; Pubmed 11687797
• Reconstitution and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Specificity: Variants (NP_001073289.1; NP_004886.3) encode the same isoform.
• Application Info: P-ELISA: Antibody detection limit dilution 1:2000
  WB: This antibody is not recommended for use in Western blotting
  IF: Strong expression of the protein seen in the plasma membrane and cytoplasm of U937 cells. Recommended concentration: 10 ug/ml.
  FC: Flow cytometric analysis of U937 cells. Recommended concentration: 10 ug/ml.
• Immunogen: NLRP3
• Uniprot ID: Q96P20
• Protein Name: NACHT, LRR and PYD domains-containing protein 3
• Protein Accession #: NP_001073289.1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Tested Species Reactivity: Human
• Gene Symbol: NLRP3
• Predicted Species Reactivity: Bovine|Human|Rat
• Application: FC, IF, WB, P-ELISA
• Image 1: Flow Cytometry
  
  Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
• Image 2: Immunofluorescence
  
  Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cyt
• Image 3: Immunohistochemistry
  
  (5ug/ml) staining of paraffin embedded Human Peripheral Blood Leukocytes. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.